摘要
目的通过生物信息学分析结合生物学实验,筛选和鉴定与心肌肥厚密切相关的枢纽基因。方法从基因表达数据库(GEO)下载小鼠心肌肥厚相关芯片数据,利用GEO2R在线工具筛选差异表达基因;利用DAVID6.7、String 11.0和Cytoscape 3.7. 0软件对差异基因进行分析;昆明小鼠随机分为生理盐水组(n=6)和血管紧张素Ⅱ(AngⅡ)组(n=6),建立心肌肥厚模型,通过Real-time PCR方法检测枢纽基因在AngⅡ诱导的昆明小鼠心肌肥厚模型中的表达。结果筛选出共有差异表达基因202个,枢纽基因12个;Real-time PCR结果显示核心蛋白聚糖(Dcn)、HADHA和热休克蛋白(HSP) 90αA1在AngⅡ组中表达明显下调。结论筛选出的枢纽基因可以通过细胞外基质和转化生长因子β(TGF-β)影响昆明小鼠心肌肥厚的发生发展。
Objective To screen and identify the hub genes closely related to cardiac hypertrophy by using bioinformaticsmethod and biological experiments.Methods The chip data related to cardiac hypertrophy in mice were downloaded from the Gene Expression Omnibus(GEO) database,and the GEO2R online tool was adopted to screen for differentially expressed genes;DAVID 6.7,String 11.0 and Cytoscape 3.7.0 softwares were used to analyze differentially expressed genes;Kunming mice were randomly divided into a normal saline group(n = 6) and an angiotensin Ⅱ(Ang Ⅱ)group(n = 6) to establish a cardiac hypertrophy model,the expression of hub gene in Kunming mouse model of cardiac hypertrophy induced by Ang Ⅱ was detected by Real-time PCR method.Results A total of 202 common differentially expressed genes and 12 hub genes were selected;the Real-time PCR result demonstrated that decorin(Dcn),HADHA and heat shock protein(HSP) 90αA1 were significantly down-regulated in the Ang Ⅱ group.Conclusion The selected hub genes can influence the development of cardiac hypertrophy in Kunming mice through extracellular matrix and transforming growth factor β(TGF-β).
作者
史卓
李家慧
高静
董艳莉
任甫
文普帅
SHI Zhuo;LI Jia-hui;GAO Jing;DONG Yan-li;REN Fu;WEN Pu-shuai(Department of Anatomy,School of Basic Medical Science,Jinzhou Medical University,Liaoning Jinzhou 121000,China;Liaoning Key Research Laboratory of Human Phenotype Group,Jinzhou Medical University,Liaoning Jinzhou 121000,China;Department of Nursing,Jinzhou Health School,Liaoning Jinzhou 121000,China;The Third Clinical Medical College,Jinzhou Medical University,Liaoning Jinzhou 121000,China;Department of Ultrasound,the First Affiliated Hospital of Jinzhou Medical University,Liaoning Jinzhou 121000,China;Nursing Group of Experimental Center,Chaoyang Health School,Liaoning Chaoyang 122000,China;Department of Pathophysiology,School of Basic Medical Science,Jinzhou Medical University,Liaoning Jinzhou 121000,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2021年第3期391-397,共7页
Acta Anatomica Sinica
基金
辽宁省科技厅自然科学基金(20170540392)
辽宁省大学生创新创业训练计划项目(201710160000187)。
