摘要
目的研究外周血循环肿瘤细胞(CTC)在乳腺癌患者中的表达及其临床意义。方法采用免疫磁珠富集阴性技术联合荧光原位杂交法检测129例乳腺癌和63例良性乳腺病患者(宜兴市肿瘤医院2017年1月至2019年12月收治)中外周血CTC的表达情况,分析两组患者外周血CTC的表达差异及CTC与乳腺癌临床病理特征的相关性。结果免疫磁珠富集阴性技术联合荧光原位杂交法检测外周血CTC的灵敏度及特异度分别为79.1%和93.7%,受试者工作特征(ROC)曲线下面积为0.881。乳腺癌患者中CTC检测阳性率为79.1%(102/129),明显高于良性乳腺病组的6.3%(4/63),P<0.05。CTC检测阳性率的高低在乳腺癌不同年龄、病理类型、临床分期及Her2表达状态中差异有统计学意义(P<0.05)。结论免疫磁珠富集阴性技术联合荧光原位杂交法检测外周血CTC具有较高的灵敏度及特异度;检测外周血CTC有助于评估乳腺癌患者的病情。
Objective To analyze the expression of circulating tumor cells(CTCs)in patients with breast cancer(BC)and its clinical significance.Methods 129 patients with breast cancer treated in Yixing Cancer Hospital from January 2017 to December 2019 were selected as the research objects.The expressions of CTCs in 129 patients with BC and 63 cases with benign breast disease were detected by immunomagnetic particle negative enrichment combined with immunofluorescence in situ hybridization.To analyze the relationship between CTCs and clinicopathologic characteristics of breast cancer.Results The sensitivity and specificity of the combined detection method for CTCs were 79.1%and 93.7%.Receiver operating characteristic curve analysis showed that the area under curve was0.881..The positive rate of peripheral venous blood CTCs in the BC group was 79.1%(102/129),which was higher than that in the control group(6.3%,4/63)significantly(P<0.05).The positive rate of CTCs were differences between the patients with different ages,pathological type,TNM stage and Her2 expression(P<0.05),and the difference was statistically significant.Conclusions Immunomagnetic particle negative enrichment combined with immunofluorescence in situ hybridization has a high sensitivity and specificity for CTCs in BC patients.The expression levels of CTCs can be used to evaluate the condition of BC patients.
作者
王建
吕旭
吴周姜丽
张涛
邵荣金
WANG Jian;LYU Xu;WU Zhoujiangli;ZHANG Tao;SHAO Rongjin(Department of Surgery, Yixing Cancer Hospital, Yixing 214200, China)
出处
《中国肿瘤外科杂志》
CAS
2021年第3期242-245,共4页
Chinese Journal of Surgical Oncology
基金
宜兴市科技创新专项资金项目(2019SF23)。
