摘要
目的探讨ApoE基因多态性及血脂水平与动脉瘤性蛛网膜下腔出血(aSAH)的关系。方法对2018年5月至2020年4月该院诊治的85例aSAH患者(病例组)和86例非aSAH患者(对照组)进行病例对照分析,采用PCR荧光探针法测定载脂蛋白E(ApoE)基因型,采用比色法和免疫比浊法测定血脂水平,使用GraphPad Prism 7统计分析ApoE基因多态性及血脂水平与aSAH的关系。结果ApoE基因型和等位基因频率在两组中差异均无统计学意义(P>0.05);病例组血清三酰甘油水平低于对照组[(1.246±0.070)mmol/L vs.(1.849±0.131)mmol/L,P<0.05];病例组中ε2/ε3型患者血清低密度脂蛋白胆固醇水平高于对照组中ε2/ε3型患者[(2.851±0.212)mmol/L vs.(2.145±0.242)mmol/L,P<0.05];病例组中ε3/ε3型患者血清三酰甘油水平低于对照组中ε3/ε3型患者[(1.216±0.080)mmol/L vs.(1.689±0.138)mmol/L,P<0.05]。结论ApoE基因多态性可能与aSAH存在关联,ApoE基因多态性可能通过血脂水平影响aSAH。
Objective To investigate the association of ApoE gene polymorphism and serum lipids levels with aneurysmal subarachnoid hemorrhage(aSAH).Methods The case-control analysis was performed on 85 cases of aSAH(case group)and 86 cases of non-aSAH(control group).The genotypes of ApoE were determined by PCR fluorescence probe,and the levels of blood lipids were determined by colorimetry and immunoturbidimetry.The statistical analysis was performed by using GraphPad Prim 7 and the relationship between ApoE gene polymorphism and blood lipids with aSAH was statistically analyzed.Results There was no statistically significant difference in ApoE genotype and allele frequency between the two groups(P>0.05);the serum triglyceride(TG)level in the case group was lower than that in the control group[(1.246±0.070)mmol/L vs.(1.849±0.131)mmol/L,P<0.05];the serum LDL-C level of the patients withε2/ε3 genotype in the case group was higher than that of the patients withε2/ε3 genotype in thecontrol group[(2.851±0.212)mmol/L vs.(2.145±0.242)mmol/L,P<0.05];the serum TG level of the patients withε3/ε3 genotype in the case group was lower than that of the patients withε3/ε3 genotype in the control group[(1.216±0.080)mmol/L vs.(1.689±0.138)mmol/L,P<0.05].Conclusion ApoE gene polymorphism may be associated with aSAH,and ApoE gene polymorphisms may affect aSAH by blood lipid levels.
作者
吴富涛
崔鲂
白垚
WU Futao;CUI Fang;BAI Yao(Department of Clinical Laboratory,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)
出处
《重庆医学》
CAS
2021年第11期1905-1908,共4页
Chongqing medicine
