摘要
提出了一种双通道探针式81°倾斜光纤光栅(81°TFG)传感器,并将其用于甲胎蛋白(AFP)的免疫检测研究。首先,在81°TFG端面镀银膜形成反射式81°TFG,构建由2×2耦合器和两支81°TFG探针组成的双通道传感器。然后,在栅区表面修饰大尺寸AuNs粒子和GO,并以AFP单克隆抗体为特异性识别单元修饰栅区表面,实现基于双通道探针式81°TFG局域表面等离子体共振(LSPR)的AFP免疫传感器。最后,在复杂血清环境下完成免疫检测实验,结果表明,该传感器对AFP抗原质量浓度的检测极限在1~10 pg/mL之间,检测的饱和点约为200 ng/mL,灵敏度约为0.155 nm/log(pg/mL),对肝癌患者的血清具有良好的特异性响应。相比单通道透射式81°TFG传感器,该传感器能实现对目标分子阳性和阴性样本的同时对照检测,从而增强临床检测应用中的可靠性,且操作更简便、应用潜力更大。
In this study,a dual-channel probe type 81°tilted fiber grating(81°TFG)sensor is proposed and used for the evaluation of alpha-fetoprotein(AFP)immunoassays.First,the end face of 81°TFG is plated with a silver film to form the reflective type 81°TFG,and a dual-channel sensor is constructed by a 2×2 coupler and two 81°TFG probes.Then,large-size AuNs and GO are modified on the grating surface,and AFP monoclonal antibodies are used as the specific recognition unit to modify the grating surface to achieve a dual-channel probe type 81°TFG local surface plasmon resonance(LSPR)AFP immunosensor.Finally,the immune detection experiment is completed in a complex serum environment.The results demonstrated that the detection limit of the sensor for the mass concentration of AFP antigen is within the range of 1--10 pg/mL,the detection saturation point and sensitivity are~200 ng/mL and~0.155 nm/log(pg/mL),respectively,it has a satisfactory specific response to the serum of liver cancer patients is observed.Compared with the single-channel transmission type 81°TFG sensor,the sensor could simultaneously control the detection of positive and negative samples of the target molecules;therefore,enhancing the reliability in clinical detection applications,and the operation is simpler and the application potential is greater.
作者
吕清明
罗彬彬
杨琪
张杨
吴胜昔
谷志鹏
石胜辉
吴德操
巫涛江
赵明富
Lu Qingming;Luo Binbin;Yang Qi;Zhang Yang;Wu Shengxi;Gu Zhipeng;Shi Shenghui;Wu Decao;Wu Taojiang;Zhao Mingfu(Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection,Chongqing University of Technology,Chongqing 400054,China;Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology,Chongqing University of Technology,Chongqing 400054,China;Chongqing Energy College,Chongqing 402247,China)
出处
《光学学报》
EI
CAS
CSCD
北大核心
2021年第7期29-38,共10页
Acta Optica Sinica
基金
国家自然科学基金(61875026)
重庆市教委科技项目(KJZD-K201901101,KJQN20181121)
重庆市自然科学基金(cstc2019jcyj-msxmX0093)
重庆理工大学研究生创新项目(ycx20192049,clgycx20202039)。
