摘要
AGAMOUS(AG)属于MADS-box基因家族成员,是与花发育调控相关的关键基因。从银杏转录组中得到AG类同源基因GBM5(登录号:AY114304.1),根据GBM5基因序列设计引物,克隆获得GBM5基因的全长cDNA序列,其大小为732 bp,编码产物含243个氨基酸。进化树及氨基酸序列比对分析表明银杏GBM5与苏铁AG、黑云杉AG亲缘关系较近。qPCR分析显示,GBM5在银杏不同组织和不同时期的表达量均有差异,不同组表达量为:雌花>雄花>茎端>叶;其在不同时期叶片和茎端表达量均为:4月>5月>9月。
AGAMOUS(AG),a member of MADS-box gene family,is a key gene related to regulation of flower development in plants.The AG homologous gene GBM5(Accession No.:AY114304.1)was obtained from the transcriptome of Ginkgo biloba.Primers were designed according to the obtained GBM5 gene sequence,and the full-length cDNA sequence of GBM5 gene with 732 bp in size and 243 amino acids in coding product can be synthetized by designing primers.The comparative analysis of evolutionary tree and amino acid sequence showed that ginkgo GBM5 was closely related to cycads AG and black spruce AG.qPCR analysis showed that the expression of GBM5 in different tissues of Ginkgo biloba was different at different periods,the expression levels of different tissues are as follows:Female flower>Male flower>Primordia>Foliage;Its expression is high in the undifferentiated stage of flower bud,the expression patterns were:April leaf>May leaf>September leaf,April primordia>May primordia>September primordia.
作者
刘艳玲
董金金
杨婷婷
李萌
杨果
冯致
詹鹏
汪贵斌
郁万文
王义强
Liu Yanling;Dong Jinjin;Yang Tingting;Li Meng;Yang Guo;Feng Zhi;Zhan Peng;Wang Guibin;Yu Wanwen;Wang Yiqiang(Hunan Provincial Key Laboratory for Forestry Biotechnology,Central South University of Forestry and Technology,Changsha,410004;Co-innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,210037;Key Lab of Non-wood Forest Nurturing and Protection of National Ministry of Education,Central South University of Forestry and Technology,Changsha,410004)
出处
《分子植物育种》
CAS
北大核心
2021年第10期3216-3222,共7页
Molecular Plant Breeding
基金
国家重点研发计划(2017YFD0600701)
国家自然科学基金(31570682)共同资助。
关键词
银杏
GBM5
基因克隆
开花调控
表达分析
Ginkgo biloba
Gene cloning
Flowering regulation
Expression analysis
