摘要
【目的】优化组培苗生根和移栽技术,提高‘黄樽’薄叶金花茶组培生根率和移栽成活率。【方法】以苗龄60 d的继代芽苗为材料,在优选基本培养基(1/2 B5、3/4 B5、B5)与植物生长调节剂(ABT、IBA)组合的基础上,研究2种培养基质[(V细河沙:V蛭石=1∶1)(河沙蛭石基质)、琼脂]与2种附加物(白糖、活性炭)形成的5种组合对生根率的影响;以河沙蛭石基质+1/2 B5+ABT0.5 mg/L+IBA 0.5 mg/L为生根培养基,培养35 d,分别补充白糖15.0 g/L、K2HPO415.0 mg/L、白糖15.0 g/L+K2HPO415.0 mg/L及对照组(CK)等4种处理,培养65 d后,将生根苗移栽到V细河沙∶V蛭石=3∶1的基质上,研究4种处理对芽苗生根率、移栽成活率、芽苗茎粗和黄化率的影响。【结果】在优选的培养基1/2B5+ABT 0.5~1.0 mg/L+IBA 0.5~1.0 mg/L+白糖15 g/L+琼脂6.0 g/L,芽苗生根率仅41.1%~42.2%;培养基质与附加物的5种组合对生根率的影响达极显著水平,河沙蛭石基质+白糖0 g/L和河沙蛭石基质+白糖15 g/L的生根率分别为84.4%和68.9%,高于传统的琼脂白糖组合;生根培养后期,4种处理对生根率的影响不显著,但对移栽成活率影响极显著,补充白糖15 g/L+K2HPO415.0 mg/L,克服了芽苗黄化,提高芽苗质量,进一步提高了芽苗生根率和移栽成活率,生根率和移栽成活率分别为91.1%和93.3%。【结论】‘黄樽’薄叶金花茶采用传统的白糖琼脂培养方式,其组培生根及组培苗移栽效果不理想。生根培养前期用河沙蛭石基质+1/2B5+ABT 0.5~1.0 mg/L+IBA 0.5~1.0 mg/L的无糖培养,培养后期补充白糖15 g/L+K2HPO415.0 mg/L,是提高‘黄樽’薄叶金花茶生根率和移栽成活率的有效方法,也为其他组培生根困难的植物,尤其是金花茶组植物离体培养,提供了可借鉴的培养方法。
【Objective】We aimed to optimize rooting and transplanting techniques to improve the transplant survival rates of tissue-cultured shoots of Camellia chrysanthoides‘Huangzun’.【Method】Tissue-cultured shoots were aged for 60 days.We optimized the content of basic media including 1/2 B5,3/4 B5 and B5,and plant growth regulators(PGRs)including ABT and IBA.Differences in rooting rates were determined among five combinations of rooting substrates and additives,including agar 6.0 g/L+sugar 0 g/L,agar 6.0 g/L+sugar 15 g/L,agar 6.0 g/L+sugar 15 g/L+charcoal(AC)3.0 g/L,substrate with 1Vsand+1Vvermiculite(sand-vermiculite substrate)+sugar 0 g/L,and sand-vermiculite substrate+sugar 15 g/L,and differences in shoot diameter,rooting,transplant survival,and yellowing rates were compared among media comprising sugar 15.0 g/L,K2HPO415.0 mg/L,sugar 15.0 g/L+K2HPO415.0 mg/L,and without sugar and K2HPO4,after the rooting-culture for 35 days,in sand-vermiculite substrate+1/2 B5+ABT 0.5 mg/L+IBA 0.5 mg/L as rooting medium,and after 65 days,with 3Vsand+1Vvermiculite as the transplant substrate.【Result】Rooting rates were 41.1%-42.2%on the optimized medium 1/2 B5+ABT 0.5-1.0 mg/L+IBA 0.5-1.0 mg/L+sugar 15 g/L+agar 6.0 g/L.The difference in rooting rates was significant among the five combinations of rooting substrates and additives;the rooting rates on sand-vermiculite substrate without sugar and sand-vermiculite substrate+sugar 15 g/L were 84.4%and 68.9%,respectively,which were higher than those on traditional combinations of agar and sugar.Rooting rates on agar 6.0 g/L+sugar 0 g/L,agar 6.0 g/L+sugar 15 g/L,agar 6.0 g/L+sugar 15 g/L+charcoal(AC)3.0 g/L,were 57.8%,40.0%and 33.3%,respectively.Rooting rates did not significantly differ among the four conditions,but transplant survival rates significantly differed.The yellowing and quality of seedlings were respectively overcome and improved,the rooting rate reached 91.1%,and the transplant survival rate reached 93.3%,when sugar 15 g/L+K2HPO415.0 mg/L was added to the medium at
作者
吴丽君
游云飞
陈达
陈文荣
李文芳
WU Lijun;YOU Yunfei;CHEN Da;CHEN Wenrong;LI Wenfang(Fujian Academy of Forestry,Fuzhou 350012,China;Yangkou Country Forest Farm,Shunchang 353211,China;The Key Lab of Southern Mountain Timber Forest Cultivation of National Forestry and Grassland Administration,Fuzhou 350012,China;The Key Lab of Forest Cultivation and Forest Products Processing and Utilization,Fuzhou 350012,China)
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第3期117-122,共6页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
福建省花卉种质创新项目(H2014018)
福建省林业科研项目(闽林科便[2017]50号)
中央财政林业示范项目(闽[2019]TG14号)
福建省林业科技推广项目(2018TG11)
福建省属公益科研项目(2021R1000-7)。
关键词
‘黄樽’薄叶金花茶
组培快繁
生根率
移栽成活率
ABT
吲哚丁酸
Camellia chrysanthoides‘Huangzun’
tissue cultur and rapid propagation
rooting rate
transplantation survival rate
technical optimization
ABT
IBA
