摘要
目的分析高危型人乳头状病毒(HPV)16感染患者阴道灌洗液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-12、IL-10表达水平与宫颈上皮内瘤变级别的相关性。方法选择2017年1月-2019年12月邢台市人民医院妇科收治的高危型HPV阳性患者300例作为研究对象,分析患者HPV型别分布,比较不同型别患者阴道灌洗液TNF-α、IL-12、IL-10表达水平差异,比较HPV16感染不同宫颈病变程度患者阴道灌洗液TNF-α、IL-12、IL-10表达水平并分析相关性。结果300例患者共检出6种高危型别,其中HPV16型别117例,占39.0%;不同HPV型别患者阴道灌洗液TNF-α、IL-12、IL-10、IL-12/IL-10水平比较差异均无统计学意义;117例HPV16感染患者根据患者宫颈病变程度分为无病变组49例,低级别鳞状上皮内病变(LSIL)组39例、高级别鳞状上皮内病变(HSIL)组21例和宫颈癌组8例。四组患者TNF-α、IL-10、IL-12表达水平比较差异有统计学意义(P<0.05);IL-10与宫颈上皮内瘤变级别呈正相关(r=0.243,P=0.041),IL-12、IL-12/IL-10与宫颈上皮内瘤变级别呈负相关(r=-0.281,-0.429,P=0.038,0.025)。结论不同HPV型别患者阴道灌洗液TNF-α、IL-12、IL-10、IL-12/IL-10水平无显著差异,HPV16感染患者阴道灌洗液中IL-12、IL-10表达与宫颈上皮内瘤变级别相关。
OBJECTIVE To observe the correlation between the expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-12,IL-10 in vaginal lavage fluid of patients with high-risk human papillomavirus(HPV)16 infection and grade of cervical intraepithelial neoplasia.METHODS A total of 300 patients who were positive for high-risk HPV and were treated in gynecology department of Xingtai People′s Hospital from Jan 2017 to Dec 2019 were recruited as the study subjects.The subtypes of HPV of the patients were analyzed,the levels of vaginal lavage fluid TNF-α,IL-12 and IL-10 were compared among the patients with different subtypes of HPV,the levels of vaginal lavage fluid TNF-α,IL-12 and IL-10 were compared among the HPV16 infection patients with different grades of cervical lesions.RESULTS Totally 6 high-risk subtypes were detected in the 300 patients,117 had HPV16,accounting for 39.0%.There were no significant differences in the levels of vaginal lavage fluid TNF-α,IL-12,IL-10 and IL-12/IL-10 among the patients with different subtypes of HPV.The 117 patients with HPV16 infection were divided into the no lesion group with 49 cases,the low-grade squamous intraepithelial lesion(LSIL)group with 39 cases,the high-grade squamous intraepithelial lesion(HSIL)group with 21 cases and the cervical cancer group with 8 cases according to the degree of cervical lesions.There were significant differences in the levels of TNF-α,IL-10 and IL-12 among the four groups(P<0.05).The IL-10 was positively correlated with the grade of cervical intraepithelial neoplasia(r=0.243,P=0.041);the IL-12 and IL-12/IL-10 were negatively correlated with the grade of cervical intraepithelial neoplasia(r=-0.281,-0.429,P=0.038,0.025).CONCLUSION There are no significant differences in the levels of vaginal lavage fluid TNF-α,IL-12,IL-10 and IL-12/IL-10 among the patients with different subtypes of HPV,and the expression of IL-12 and IL-10 in the vaginal lavage fluid of the patients with HPV16 infection is associated with the grade of cervical intraepithelial
作者
申鑫鑫
豆贝贝
滑天
邱红兵
邱文娜
SHEN Xin-xin;DOU Bei-bei;HUA Tian;QIU Hong-bing;QIU Wen-na(Xingtai People′s Hospital,Xingtai,Hebei 054000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2021年第9期1419-1422,共4页
Chinese Journal of Nosocomiology
基金
河北省医学科学研究重点基金资助项目(20180887)。
