摘要
目的探讨慢性乙型肝炎(CHB)患者外周血滤泡辅助性T细胞(Tfh)相关表达分子CXCR5、CXCL13、IL-21和TGF-β_(2)的表达水平,观察自身抗体出现的频率,分析其关联性。方法选取2018年10月~2019年3月广州中医药大学第一附属医院收治的CHB患者50例,再选取同期健康体检人员30例作为对照组,采用ELISA法检测两组受试者外周血中CXCR5、CXCL13、IL-21和TGF-β_(2)的表达水平,间接免疫荧光法检测ANA、LKM抗体、LC1抗体、ASMA和AMA等自身抗体的效价,ANA同时区分核型。结果CHB患者组CXCR5、CXCL13、IL-21和TGF-β_(2)表达明显高于健康对照组(P<0.01),自身抗体阳性率也明显高于健康对照组(P<0.05),ANA阳性荧光核型表现为核颗粒型7例,胞质颗粒型1例,胞质纤维型1例和1例混合核型(核仁型和核颗粒型),ASMA阳性率为6%,未检出AMA抗体、LC1抗体和LKM抗体。CHB患者自身抗体阳性组的CXCL13、CXCR5、TGF-β_(2)和IL-21表达与阴性组比较差异无统计学意义(P>0.05)。结论Tfh细胞相关表达分子CXCL13、CXCR5、TGF-β_(2)和IL-21在CHB患者中高表达,CHB患者体内存在明显自身免疫紊乱现象,但CHB患者体内自身抗体的出现与CXCL13、CXCR5、TGF-β_(2)和IL-21的关联性并不强,有待于更深入研究。
Objective Investigate the expression levels of CXCR5,CXCL13,IL-21 and TGF-β_(2) related expression molecules of peripheral blood Tfh cells in patients with chronic hepatitis B(CHB)and to analyze their correlation with autoantibodies.Methods Fifty CHB patients admitted to the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from October 2018 to March 2019 were selected.30 healthy medical examiners during the same period were selected as the control group.The expression levels of CXCR5,CXCL13,IL-21 and TGF-β_(2) were detected by ELISA method,and the titer of autoantibodies such as ANA,LKM,LC1,ASMA,AMA,and etc.are obtained by indirect immunofluorescence method.ANA are also distinguished karyotypes.Results The expression of CXCR5,CXCL13,IL-21 and TGF-β_(2) in the CHB patient group was significantly higher than that of the healthy control group(P<0.01),and the positive rate of autoantibodies was also significantly higher than that of the healthy control group(P<0.05).Under ANA positive fluorescent karyotype,six cases of nuclear granular type and one case of cytoplasmic particle type,one case of cytoplasmic fiber type and one case of mixed karyotype(nuclear type and nuclear granular type)were present.The positive rate of ASMA was 6%,which had no AMA,LC1 and LKM presenting.CXCL13 and CXCR5 in the CHB patients′autoantibody positive group were slightly higher than the negative group,and IL-21 was slightly lower than the negative group.The differences of CXCL13,CXCR5,and IL-21 were not statistically significant(P>0.05).Conclusion Tfh cells expression molecules CXCL13,CXCR5,TGF-β_(2) and IL-21 were highly expressed in CHB patients.There were obvious autoimmune disorders in CHB patients.The correlation between the appearance of autoantibodies in CHB patients and the expression molecules of Tfh cells CXCL13,CXCR5,TGF-β_(2) and IL-21 was not strong enough which requires further study.
作者
梁淑慧
黄燕晓
李翠
孙六娜
邓日辉
黄惠
周迎春
Liang Shuhui;Huang Yanxiao;Li Cui(The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangdong 510405,China)
出处
《医学研究杂志》
2021年第5期47-50,共4页
Journal of Medical Research
基金
广东省科技计划项目(2017ZC0142)。
