摘要
【目的】研究OfNAC基因对桂花Osmanthus fragrans花开放的调控作用。【方法】从桂花品种‘堰虹桂’O.fragrans ‘Yanhonggui’转录组数据中,筛选获得相关OfNAC基因序列,分析预测其理化性质和结构,运用实时荧光定量PCR技术分析花开放过程的表达特性。【结果】筛选得到22条OfNAC序列。生物信息学分析发现:22条OfNAC转录因子均含有NAM结构域,氨基酸序列含有5个保守的亚结构域(A~E),其保守性由强到弱依次为C、A、D、B、E;二级结构中不同结构的占比由大到小表现为无规则卷曲、α-螺旋、延伸链、β-折叠;亚细胞定位及跨膜结构预测表明:OfNAC17、OfNAC17-X2、OfNAC53、OfNAC91、OfNTM1-9是膜结合转录因子,且大多数OfNAC定位在细胞核。在桂花花开放进程中,OfNAC100-2、OfNAC43、OfNAC73相对表达量在铃梗期(S4)到达顶峰,在此之后相对表达降低;OfNAC43在铃梗期(S4)骤然升高,并且在此时期相对表达最大;OfNAC71、OfNAC29-1、OfNAC21/22从起始期(S1)呈缓慢上升趋势,在顶壳期(S3)到达最高,随后整体呈现下降趋势;OfNAC29-2在圆珠期(S2)相对表达量陡然上升,在铃梗期(S4)相对表达最低。【结论】推测OfNAC100-2、 OfNAC43、 OfNAC73、 OfNAC71、 OfNAC29-1、 OfNAC21/22、OfNAC29-2等成员极有可能参与调控桂花的花开放。
[Objective] This study aims to investigate the regulation of OfNAC genes on flower opening of Osmanthus fragrans. [Method] From the transcriptome data of O. fragrans ‘Yanhonggui’, the related OfNAC genes were screened and analyzed to predict the physicochemical properties and structure. Real-time fluorescence quantitative PCR was used to analyze the expression characteristics of the flower opening process.[Result] 22 OfNAC sequences were screened from transcriptome. Bioinformatics analysis showed that all of the 22 OfNAC transcription factors contained NAM domains, and the amino acid sequence contained 5 conserved subdomains(A-E). The sequence of conservatism ranging from strong to weak was C, A, D, B, andE. The proportion of different structures in the secondary structure from large to small was random coils, α-helices, extended strands, and β-sheets. Subcellular localization and transmembrane structure prediction showed that Of NAC17, Of NAC17-X2, Of NAC53, Of NAC91, Of NTM1-9 were membrane-bound transcription factors, and most Of NACs were located in the nucleus. During the O. fragrans flowering process, the relative expression levels of Of NAC100-2, Of NAC43 and Of NAC73 reached the peak at the boll stem stage(S4), and then decreased. The relative expression of Of NAC43 was the highest at the boll stem stage(S4). The relative expression of Of NAC71, Of NAC29-1, and Of NAC21/22 increased slowly from the initial stage(S1), reached the highest at the apical shell stage, and then decreased as a whole. The relative expression of Of NAC29-2 increased sharply at the bead stage(S2) and reached the lowest at the boll stem stage(S4). [Conclusion] It is speculated that Of NAC100-2, Of NAC43, Of NAC73, Of NAC71, Of NAC29-1, Of NAC21/22, Of NAC29-2 may be involved in the regulation of O. fragrans flower opening.
作者
缪云锋
周丹
董彬
赵宏波
MIAO Yunfeng;ZHOU Dan;DONG Bin;ZHAO Hongbo(Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;College of Landscape Architecture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2021年第3期433-444,共12页
Journal of Zhejiang A&F University
基金
国家自然科学基金面上项目(32072615)。
关键词
植物学
桂花
花开放
NAC转录因子
表达分析
botany
Osmanthus fragrans
flower opening
NAC transcription factor
expression analysis
