摘要
采用多种柱色谱技术方法对喜热灵芝子实体乙醇提取物中化合物进行分离纯化,通过波谱数据鉴定其结构,并检测化合物抗肿瘤活性。结果分离得到11个化合物,分别鉴定为(24E)-lanosta-8,24-dien-3, 11-dione-26-al(1)、ganoderone A(2)、3-oxo-15α-乙酰氧基-羊毛甾-7,9(11),24-三烯-26-油酸(3)、(23E)-27-nor-羊毛甾-8,23-二烯-3,7,25-三酮(4)、ganodecalone B(5)、ganoderic aldehyde A(6)、11β-hydroxy-lucidadiol(7)、3,4-二羟基苯乙酮(8)、龙胆酸甲酯(9)、ganoleucin C(10)和ganot-heaecolumol H(11),其中化合物1为1个新的三萜类化合物。抗肿瘤活性测定结果表明化合物1,3,4和6对肝癌BEL-7402细胞株具有细胞毒活性,其IC50分别为26.55, 11.35, 23.23, 18.66μmol·L^(-1);化合物1和3~6对白血病K562具有细胞毒活性,其IC50分别为5.79, 22.16, 12.16, 35.32和5.59μmol·L^(-1);化合物4对肺癌A549具有细胞毒活性,其IC_(50)为42.50μmol·L^(-1)。
Chemical constituents were isolated and purified from fruiting bodies of Ganoderma calidophilum by various column chromatographic techniques, and their chemical structures were identified through combined analysis of physicochemical properties and spectral data. As a result, 11 compounds were isolated and identified as(24E)-lanosta-8,24-dien-3,11-dione-26-al(1), ganoderone A(2), 3-oxo-15α-acetoxy-lanosta-7,9(11), 24-trien-26-oleic acid(3),(23E)-27-nor-lanosta-8,23-diene-3,7,25-trione(4), ganodecanone B(5), ganoderic aldehyde A(6), 11β-hydroxy-lucidadiol(7), 3,4-dihydroxyacetophenone(8), methyl gentiate(9), ganoleucin C(10), ganotheaecolumol H(11). Among them, compound 1 is a new triterpenoid. The cytotoxic activities of all of the compounds against tumor cell lines were evaluated. The results showed that compounds 1, 3, 4 and 6 showed cytotoxic activity against BEL-7402, with IC50 values of 26.55, 11.35, 23.23, 18.66 μmol·L^(-1);compounds 1 and 3-6 showed cytotoxic activity against K562, with IC50 values of 5.79, 22.16, 12.16, 35.32, and 5.59 μmol·L^(-1), and compound 4 showed cytotoxic activity against A549, with IC_(50) value of 42.50 μmol·L^(-1).
作者
张婷婷
郭教岑
马青云
孔凡栋
周丽曼
谢晴宜
戴好富
郁志芳
赵友兴
ZHANG Ting-ting;GUO Jiao-cen;MA Qing-yun;KONG Fan-dong;ZHOU Li-man;XIE Qing-yi;DAI Hao-fu;YU Zhi-fang;ZHAO You-xing(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Haikou Key Laboratory for Research and Utilization of Tropical Natural Products,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Hainan Academy of Tropical Agricultural Resource,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第7期1783-1789,共7页
China Journal of Chinese Materia Medica
基金
海南省自然科学基金项目(219MS078)
国家自然科学基金项目(81973568)
现代农业产业技术体系建设专项(CARS-21)
农业农村部财政专项(NFZX2018)
中国热带农业科学院基本科研业务费专项(17CXTD-15,1630052016008)。
关键词
喜热灵芝
化学成分
抗肿瘤活性
Ganoderma calidophilum
chemical constituents
cytotoxic activity
