摘要
为了明确宁夏地区奶牛犊牛腹泻源性大肠埃希氏菌的分离率、耐药情况和LEE毒力岛eaeA和ler基因的携带情况,采集宁夏5市16个规模化奶牛场1~2月龄犊牛病理性腹泻样本157份,采用微生物学方法培养、VITEK 2 Compact生化鉴定和PCR鉴定,应用PCR方法进行分离株LEE相关基因检测。结果分离鉴定到大肠埃希氏菌62株,对四环素耐药率为100%,对阿莫西林、氟苯尼考、哌拉西林等9种药物的耐药率大于90.00%,对阿米卡星较为敏感,耐药率为11.29%;LEE毒力岛eaeA基因的检出率为11.29%(7/62),ler基因的检出率为79.03%(49/62),二者同时检出率为3.23%(2/62)。结果表明,LEE的存在,可能是某些奶牛场犊牛腹泻大肠埃希氏菌病的原因之一,建议临床治疗犊牛腹泻大肠埃希氏菌病可以首选阿米卡星。
The purpose of this study was to determine the isolation rate,drug resistance and the carrying of eaeA and ler LEE related genes of E.coli from diarrhea calves(1-2 months)in Ningxia.157 anal swab samples were collected from 16 scale dairy farms in 5 cities cultured by microbiology,identified by VITEK 2 compact biochemistry and PCR methods.LEE-related genes were detected by PCR.The results showed that 62 strains of E.coli were isolated,The drug resistance rate of the isolates to tetracycline was 100%,to amoxicillin,florfenicol,pipracil and other nine kinds of drugs were>90%,and sensitive to amikacin,the resistance rate was 11.29%.Detection rate of eaeA and ler genes were 11.29%(7/62)and 79.03%(49/62)respectively,both simutaneous detection rate was 3.23%(2/62).In conclusion,one of the reasons caused calves diarrhea by E.coli was carrying LEE,clinical treatment of diarrhea calves caused by E.coli can choose amikacin first.
作者
高海慧
王建东
高小斐
王培柱
吴学青
厉龙
康晓冬
GAO Hai-hui;WANG Jian-dong;GAO Xiao-fei;WANG Pei-zhu;WU Xue-qing;LI Long;KANG Xiao-dong(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002,China;Animal Husbandry and Veterinary Station,Fanxue Town,Dingbian,Shaanxi 718699,China;Animal Husbandry and Veterinary Station,Biandangou Town,Litong,Wuzhong,Ningxia 751100,China;Animal Disease Prevention and Control Center of Huinong of Shizuishan City,Luoping,Ningxia 753200,China)
出处
《动物医学进展》
北大核心
2021年第6期19-23,共5页
Progress In Veterinary Medicine
基金
宁夏农林科学院农业科技自主创新项目(NKYQ-20-01)(NKYJ-20-12)
农业高质量发展和生态保护科技创新示范专项(NGSB-2021-12-06)
宁夏农林科学院平台提升项目(NKYP-19-14)。
