摘要
目的探讨结直肠癌(CRC)患者血清microRNA-497(miR-497)表达量与CRC侵袭转移及预后的关系。方法实时荧光定量PCR(qRT-PCR)检测93例CRC患者术前及术后、30例结直肠腺瘤息肉患者及30位健康者血清miR-497表达量,同时化学发光法检测血清癌胚抗原(CEA)含量;随访CRC患者术后3年预后,复测血清miR-497表达量;分析术前血清miR-497表达量与CRC临床病理特征及预后的关系。结果CRC组术前血清miR-497表达量显著低于其术后治疗结束时、腺瘤息肉组和正常对照组(P<0.01),CEA含量显著高于术后治疗结束时、结直肠腺瘤息肉组和正常对照组(P<0.01);结直肠癌组术后治疗结束时血清miR-497表达量低于正常对照组(P<0.05),血清CEA含量高于术后正常对照组(P<0.05);CRC组术前血清miR-497诊断阳性率显著高于血清CEA(P<0.01);复发转移组术前及术后血清miR-497表达量均显著低于无复发转移组(P<0.01);术前血清miR-497表达量与CRC分化程度、TNM分期、是否淋巴结转移、是否远处转移、生存期长短有关(P<0.01),而与患者年龄、性别、肿瘤大小及部位均无相关性(P>0.05)。Cox多因素分析显示肿瘤分化程度、TNM分期、术前血清miR-497表达量、淋巴结转移及远处转移均是影响CRC患者预后的独立危险因素(P<0.05);血清miR-497低表达量组1、2、3年生存率低于血清miR-497高表达量组(P<0.05)。结论术前血清miR-497低表达与CRC侵袭转移、不良预后密切相关,可成为其预后评价指标;术后血清miR-497表达量降低对CRC术后复发转移有一定预测价值。
Objective To investigate the correlation between microRNA-497(miR-497)expression and invasion and metastasis and prognosis of colorectal cancer(CRC).Methods The expression of miR-497 in 93 CRC patients(CRC group),30 colorectal adenoma polyps(colorectal adenomatous polyp group)and 30 healthy patients(normal control group)were detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR);meanwhile,the content of serum carcinoembryonic antigen(CEA)was detected by chemiluminescence;After 3 years follow-up,the expression of miR-497 was measured again;the correlation between the expression of miR-497 and the clinicopathological characteristics and prognosis of CRC was analyzed.Results The expression of miR-497 in CRC group was significantly lower than that at the end of postoperative treatment,adenomatous polyps group and normal control group(P<0.01),and the content of CEA in CRC group was significantly higher than that at the end of postoperative treatment,adenomatous polyps group and normal control group(P<0.01);At the end of postoperative treatment,the expression of serum miR-497 in CRC group was lower than that in normal control group(P<0.05),and the content of serum CEA was higher than that in normal control group(P<0.05).The diagnosis positive rate of serum miR497 expression in CRC group was significantly higher than that of serum CEA(P<0.01),and they showed no correlation(r=0.232,P>0.05).The pre-and post-operative serum miR-497 expression levels in the recurred and metastasis group were significantly lower than those in the no postoperative recurrence and metastasis group(P<0.01).The preoperative expression of miR-497 was related to the differentiation degree of CRC,tumor node metastasis(TNM)stage,lymph node metastasis,distant metastasis and survival period(P<0.01),but not with the age,sex,tumor size and location of the patients(P>0.05).Cox multivariate analysis showed that tumor differentiation,TNM stage,preoperative miR-497 expression,lymph node metastasis and distant metastasis were all i
作者
刘少平
卫银芝
胡亚华
张海
章国星
Liu Shaoping;Wei Yinzhi;Hu Yahua;Zhang Hai;Zhang Guoxing(Department of General Practice of Huangshi Central Hospital Affiliated to Hubei Polytchnic University,Edong Healthcare Group,Huangshi 435000,China;Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology,Wuhan 430062,China;Department of Gastroenterology of Huangshi Central Hospital Affiliated to Hubei Polytchnic University,Edong Healthcare Group,Huangshi 435000,China)
出处
《中国医师杂志》
CAS
2021年第5期711-715,共5页
Journal of Chinese Physician
基金
湖北省卫生健康科研基金项目(WJ2019H184)。
