摘要
目的检测肾移植受者不同免疫状态外周血中髓源性抑制细胞(MDSC)的水平,探讨肾移植受者外周血中MDSC的变化规律及MDSC在免疫状态评估中的作用。方法收集2016年10月至2017年5月于郑州大学第一附属医院肾移植科行肾移植手术的30例受者术前及术后1、7、14、30、90 d的外周血单个核细胞,采用流式细胞术检测MDSC的细胞比例;以健康正常人作为对照,流式细胞术检测肾移植术后肾功能稳定组、肺部感染组和急性排斥组外周血MDSC比例,采用两独立样本t检验和单因素方差分析统计学方法进行分析。结果以移植术前1 d水平[(2.02±1.90)%、(0.39±0.30)%、(1.63±1.48)%]作为基线,肾移植受者外周血MDSC、单核样髓源性抑制细胞(Mo-MDSC)、粒细胞样髓源性抑制细胞(G-MDSC)水平均于术后1 d大幅度上升[(6.76±4.85)%,(1.21±0.85)%,(5.55±3.01)%]且显著高于术前水平(P<0.05),达到峰值后细胞水平呈现下降趋势,术后30 d时降至术前水平[(2.01±1.84)%、(0.53±0.36)%、(1.49±1.01)%],随后呈现缓慢上升趋势,至90 d时MDSC比例高于移植前水平[(2.34±1.38)%、(0.68±0.39)%、(1.67±1.01)%],差异无统计学意义(P>0.05);肾移植受者Mo-MDSC于术后1 d显著低于对照供者[(1.21±0.85)%比 (2.19±1.08)%,t=2.95,P<0.05],术后14 d显著高于对照供者[(0.56±0.48)%比 (0.31±0.21)%,t=1.586,P<0.05];G-MDSC术后14 d显著高于对照供者[(1.99±1.01)%比 (1.04±0.46)%,t=2.885,P<0.05],其他时间点两组间比较差异无统计学意义;相较于正常人[(1.79±1.48)%、(0.34±0.29)%]、尿毒症患者[(1.92±1.58)%、(0.39±0.29)%]及移植肾功能稳定组受者[(2.36±2.30)%、(0.62±0.42)%],肾移植术后感染组患者外周血中MDSC与Mo-MDSC[(3.80±3.47)%、(1.90±1.72)%]比例显著升高,差异有统计学意义(P<0.05),G-MDSC两两间的差异无统计学意义(F=0.238,P>0.05);急性排斥反应组患者外周血MDSC与G-MDSC[(1.28±1.18)%、(0.89±0.75)%]明显降低,与术后肾功能稳定组�
Objective To explore the changes of myeloid-derived suppressor cells(MDSCs)in renal transplant recipients under different immune states and to analyze the correlation between those cells,so as to assess their roles under different immune states in renal transplantation.Methods The peripheral blood was collected in 30 recipients undergoing renal transplantation in our hospital from October 2016 to May 2017,at the 1st day before surgery and 1st,7th,14th,30th and 90th day after surgery.Flow cytometry was used to detect the dynamic changes of MDSCs.Flow cytometry was used to compare the levels of MDSCs in peripheral blood of stable graft function group,pathogen infection group and acute rejection group after renal transplantation.Two independent samples t test and one-way analysis of variance statistical methods were used for analysis.Results Taking the level at 1st before transplantation[(2.02±1.90)%,(0.39±0.30)%,(1.63±1.48)%]as the baseline,the proportion of MDSCs,monocyte MDSCs(Mo-MDSC),and granulocyte-MDSCs(G-MDSCs)in peripheral blood increased significantly[(6.76±4.85)%,(1.21±0.85)%,(5.55±3.01)%]at 1st day after transplantation(all P<0.05).After reaching the peak,the proportion of cells showed a downward trend,and dropped to the preoperative level[(2.01±1.84)%,(0.53±0.36)%,(1.49±1.01)%]at 30th day after surgery,and then showed a slow upward trend.At 90th day after transplantation,the proportion of MDSCs was higher than the pre-transplant level[(2.34±1.38)%,(0.68±0.39)%,(1.67±1.01)%],but the difference was not statistically significant(P>0.05);The proportion of Mo-MDSCs at 1st day after transplantation in kidney transplant recipients was lower than that in the control donor[(1.21±0.85)%vs.(2.19±1.08)%,t=2.95,P<0.05],and that on the postoperative day 14 was significantly higher than in the control donor[(0.56±0.48)%vs.(0.31±0.21)%,t=1.586,P<0.05];The proportion of G-MDSCs on the postoperative day 14 was significantly higher than that in control donors[(1.99±1.01)%vs.(1.04±0.46)%,t=2.885,P<0.05],a
作者
刘佳
孙佳佳
李晓虎
岳文龙
王莹
李金锋
Liu Jia;Sun Jiajia;Li Xiaohu;Yue Wenlong;Wang Ying;Li Jinfeng(Department of Diagnostics,Henan Medical College,Zhengzhou 451191,China;Department of Kidney Transplantation,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《中华实验外科杂志》
CAS
北大核心
2021年第5期949-953,共5页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(82070771)
河南省医学科技攻关计划项目(SBGJ2018022)
河南省医学教育研究项目(Wjlx2020057)。
关键词
肾移植
免疫状态
Renal transplantation
Immune state
