摘要
目的评价免疫学技术检测日本血吸虫感染病人和病畜经吡喹酮治疗后血清抗血吸虫抗体转归情况。方法检索中国知网、万方数据、PubMed、ScienceDirect等数据库,筛选1991—2020年公开发表的关于日本血吸虫病免疫学检测的文献,提取纳入研究文献相关数据,使用Rev Man 5.3统计软件绘制漏斗图分析发表偏倚,采用meta分析进行综合评价。结果最终纳入40篇文献,其中中文文献33篇、英文文献7篇,均为采用间接血凝试验(IHA)或酶联免疫吸附试验(ELISA)开展的研究。效应量合并分析结果显示,吡喹酮治疗后6个月内,ELISA和IHA法检测血清抗日本血吸虫抗体阴性率分别为45.36%[95%可信区间(CI):(43.96%,46.76%)]和20.83%[95%CI:(19.69%,21.97%)];治疗后6~12个月,ELISA和IHA法检测抗体阴性率分别为62.95%[95%CI:(61.59%,64.31%)]和55.61%[95%CI:(54.21%,57.01%)];治疗12个月后,ELISA和IHA法检测抗体阴性率分别为85.92%[95%CI:(84.94%,86.90%)]和86.90%[95%CI(85.95%,87.85%)]。结论随着吡喹酮治疗后时间推移,IHA、ELISA法检测血清抗日本血吸虫抗体阴性率升高。在治疗后12个月内,2种方法检测血清抗日本血吸虫抗体阴性率总体较低,但在治疗12个月后如无新的疫水接触史,抗体阴性率较高。
Objective To evaluate the conversion of serum antibodies against Schistosoma japonicum in humans and livestock detected by immunological tests following treatment with praziquantel.Methods The studies pertaining to serological tests of schistosomiasis japonica published from 1991 to 2020 were retrieved in electronic databases,including Chinese National Knowledge Infrastructure,WanFang Data,PubMed and ScienceDirect.Data were extracted from included studies.The publication bias was assessed with funnel plots using the software RevMan version 5.3,and the conversion of antibodies against S.japonicum was evaluated through meta-analysis.Results A total of 40 publications were included in the final meta-analysis,consisting of 33 Chinese publications and 7 English publications,and all immunological tests were performed with indirect hemagglutination test(IHA) and enzyme-linked immunosorbent assay(ELISA).Pooled analysis showed that the negative rates of serum anti-S.japonicum antibody were 45.36% [95% confidential interval(CI):(43.96%,46.76%)] and 20.83% [95% CI:(19.69%,21.97%)] detected by ELISA and IHA within 6 months post praziquantel treatment,62.95% [95% CI:(61.59%,64.31%)] and 55.61% [95% CI:(54.21%,57.01%)] within 6 to 12 months after treatment and 85.92% [95% Cl:(84.94%,86.90%)] and 86.90% [95% CI:(85.95%,87.85%)] over 12 months after treatment,respectively.Conclusions The negative rate of the serum anti-S.japonicum antibody by IHA and ELISA increased with the time of post-treatment with praziquantel.The overall negative rates of anti-S.japonicum antibody detected by IHA and ELISA are low within 12 months post praziquantel treatment.However,a high negative rate of anti-S.japonicum antibody is detected if there is no new contact with infested water after 12 months of praziquantel treatment.
作者
王丽萍
邓王平
贾铁武
秦志强
许静
WANG Li-Ping;DENG Wang-Ping;JIA Tie-Wu;QIN Zhi-Qiang;XU Jing(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),NHC Key Laboratory of Parasite and Vector Biology,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Shanghai 200025,China)
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2021年第2期138-147,共10页
Chinese Journal of Schistosomiasis Control
基金
国家科技重大专项(2018ZX10101002-002)
国家自然科学基金(82073619)。
关键词
日本血吸虫病
吡喹酮
抗体转归
免疫学检测
META分析
Schistosomiasis japonica
Praziquantel
Antibody conversion
Immunological test
Meta-analysis
