摘要
目的:研究骨形态发生蛋白-2(BMP-2)对放射性损伤后骨髓间充质干细胞(BMSCs)向成纤维分化的抑制作用及分子机制。方法:建立BMSCs细胞放射损伤模型,选择最佳BMP-2干预剂量;体外培养BMSCs,随机分为对照组、放射组、BMP-2组、BMP-2+头蛋白组(Noggin)。利用Western blot、免疫荧光技术和RT-PCR等方法检测不同组别α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原(Col-Ⅰ)、Ⅲ型胶原(Col-Ⅲ)及转化生长因子β1(TGF-β1)、Smad2/3、p-Smad2/3等蛋白及分子的表达变化情况。结果:放射组BMSCs中α-SMA、Col-Ⅰ、Col-Ⅲ、TGF-β1及p-Smad2/3的表达较对照组明显增高(P<0.05),使用BMP-2干预后其表达量较放射组明显下降(P<0.05),Noggin组表达量与放射组无统计学差异。结论:BMP-2可抑制放射损伤后BMSCs向成纤维细胞分化,其机制可能与TGF-β1/Smad途径密切相关。
Objective:To study the inhibitory effects of BMP-2 on the differentiation of bone marrow mesenchymal stem cells(BMSCs)into myofibroblasts after radiation injury and to explore the related molecular mechanism.Methods:Radiation injury model of BMSCs was established.BMSCs were randomly divided into control group,radiation group(radiation alone),BMP-2 group(adding BMP-2 after radiation)and BMP-2+Noggin group.The expression ofα-smooth muscle actin(α-SMA),type I collagen(Col-Ⅰ),type III collagen(Col-Ⅲ),transforming growth factor-β1(TGF-β1),Smad2/3,p-Smad2/3 were detected in all groups by Western blot,immunofluorescence technique and RT-PCR respectively.Results:The expression ofα-SMA,Col-I,Col-III,TGF-β1 and p-Smad 2/3 in radiation group were significantly higher than that in control group(P<0.05).After the intervention of BMP-2,the expression ofα-SMA,Col-I,Col-III,TGF-β1 and p-Smad2/3 were decreased(P<0.05).However,there was no statistical difference between BMP-2+Noggin group and radiation group.Conclusion:BMP-2 can inhibit the differentiation of BMSCs into myofibroblasts after radiation injury,and its mechanism may be related to TGF-β1/Smads pathway.
作者
唐子豪
宗春琳
张维文
赵路
田磊
李德超
TANG Zihao;ZONG Chunlin;ZHANG Weiwen;ZHAO Lu;TIAN Le;LI Dechao(School of Stomatology,Jiamusi University,Jiamusi 154007,China;State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,Xi'an;Qingdao Stomatological Hospital Affiliated to Qingdao University)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2021年第3期312-316,共5页
Journal of Practical Stomatology
基金
国家自然科学基金青年项目(编号:81903249)
国家自然科学基金面上项目(编号:81670964)
黑龙江省教育厅课题(编号:2018-KYYWF-0934)
陕西省自然科学基础研究计划一般项目(编号:2019JQ-701)。
