摘要
钙蛋白酶(calpain)是一类依赖Ca^(2+)调控的半胱氨酸蛋白酶,广泛存在于生物体内,在细胞增殖、周期调控与凋亡以及糖转运、信号转导等过程中发挥重要作用。植物编码独特的钙蛋白酶,称为DEFECTIVE KERNEL1 (DEK1)。由于其缺乏经典钙蛋白酶的结构域DIV (domain IV),故被称为非经典钙蛋白酶。DEK1基因首先在玉米中克隆到,被认为参与调控糊粉层发育;后来,该基因又被证明在拟南芥、苔藓、番茄等陆地植物中存在,且其序列和功能高度保守。此外, DEK1还在调控植物胚胎发育、营养器官发育、生殖发育以及胁迫响应等方面发挥重要作用。本文综述了植物钙蛋白酶的发现以及其结构与功能,为今后更深入地开展植物钙蛋白酶的功能研究提供参考。
The widely distributed calpain belongs to a class of Ca^(2+)-dependent cysteine protease, whose function requires calcium ions. Calpain has been shown to play vital roles in cell proliferation, cell cycle regulation, apoptosis, sugar transport, and signal transduction. Plants encode a unique calpain called DEFECTIVE KERNEL1(DEK1). DEK1 lacks the typical calpain DIV domain, therefore it is considered as an atypical calpain. It was initially cloned in maize to have a role in aleurone development. Later, the DEK1 genes were also identified in other plants, including Arabidopsis, moss, and tomato, and they exhibit highly conserved sequences and functions across different plant species. DEK1 is also shown to play important roles in embryo development, vegetative and reproductive development, and the response to stress. Herein, we summarized the discovery of phytocalpain, its structure and function in plants with an aim to provide a reference for further study of the plant calpain in the future.
作者
丁竞
郭长奎
DING Jing;GUO Changkui(School of Agriculture and Food Science,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2021年第3期513-520,共8页
Plant Physiology Journal
基金
国家自然科学基金(31770352)
浙江省自然科学基金(LY20C020002)。
关键词
植物钙蛋白酶
结构
功能
研究进展
phytocalpain
structure
function
research progress
