摘要
目的探讨高危型人乳头瘤病毒(HR-HPV)感染者宫颈免疫状况及与宫颈癌的关系。方法选取2018年6月至2020年10月本院收治的104例HR-HPV感染患者,其中宫颈癌筛查正常人群39例(正常组)、宫颈高级别上皮内瘤变36例(HSIL组)、宫颈癌29例(宫颈癌组),比较3组一般资料、CD3^(+)T细胞、CD4^(+)T细胞、CD8^(+)T细胞、调节性T细胞(Treg),采用Pearson、Logistic回归方程、受试者工作特征曲线(ROC)及ROC下面积(AUC)对数据进行统计分析。结果3组HR-HPV载量、CD8^(+)T细胞、Treg比较:宫颈癌组>HSIL组>正常组,差异有统计学意义(P<0.05);CD3^(+)T细胞、CD4^(+)T细胞比较:宫颈癌组<HSIL组<正常组,差异有统计学意义(P<0.05);3组CD3^(+)T细胞、CD4^(+)T细胞与HR-HPV载量呈负相关(P<0.05),CD8^(+)T细胞、Treg与HR-HPV载量呈正相关(P<0.05);CD3^(+)T细胞、CD4^(+)T细胞、CD8^(+)T细胞、Treg高于均值者发生宫颈癌的风险分别是低于均值的0.657、0.491、3.339、4.054倍(P<0.05);CD3^(+)T细胞、CD4^(+)T细胞、CD8^(+)T细胞、Treg预测宫颈癌的AUC依次为0.850、0.740、0.825、0.799,各指标联合预测宫颈癌的AUC为0.935(P<0.05)。结论高危型HPV感染者宫颈局部细胞免疫功能异常与宫颈癌发生显著相关,有望成为预测宫颈癌发生的新型分子标志物。
Objective To explore the relationship between cervical immune status and cervical cancer in patients with high-risk human papillomavirus(HR-HPV)infection.Methods From June 2018 to October 2020,104 patients with HR-HPV infection were selected in our hospital.Among them,39 cases of normal people(normal group)were detected by cervical cancer screening,36 cases of cervical high-grade intraepithelial neoplasia(HSIL group),and 29 cases of cervical cancer(cervical cancer group).The general data of the 3 groups,CD3^(+)T cells,CD4^(+)T cells,CD8^(+)T cells,and regulatory T cells(Treg)were compared.Pearson,Logistic regression equation,receiver operating characteristic curve(ROC)and area under ROC(AUC)were used for statistical analysis.Results HR-HPV load,CD8^(+)T cells,Treg:cervical cancer group>HSIL group>normal group(P<0.05).CD3^(+)T cells,CD4^(+)T cells:cervical cancer group<HSIL group<normal group(P<0.05).CD3^(+)T cells and CD4^(+)T cells were negatively correlated with HR-HPV load,and CD8^(+)T cells,Treg and HR-HPV load were positively correlated(P<0.05).The risk of cervical cancer with higher than average CD3^(+)T cells,CD4^(+)T cells,CD8^(+)T cells,and Tregs was 0.657,0.491,3.339,4.054 times lower than the average(P<0.05).The AUC of cervical cancer predicted by CD3^(+)T cells,CD4^(+)T cells,CD8^(+)T cells and Treg were 0.850,0.740,0.825,0.799,respectively,and the AUC of each index combined to predict cervical cancer was 0.935(P<0.05).Conclusion Cervical cellular immune function abnormalities in patients with high-risk HPV infection are significantly related to the occurrence of cervical cancer,and it is expected to become a new molecular marker for predicting the occurrence of cervical cancer.
作者
程文俊
申庆文
CHENG Wenjun;SHEN Qingwen(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui,China,233000)
出处
《分子诊断与治疗杂志》
2021年第5期766-769,共4页
Journal of Molecular Diagnostics and Therapy
基金
安徽省科技项目(KJ2019A0343)。
