摘要
为明确细菌在松材线虫生态适应性中的作用,本研究选择与致病相关的松材线虫伴生细菌GD1、马尾松内生细菌GD2以及具有杀松材线虫活性的湿地松内生细菌NJSZ-13为试验对象,测定经这3株芽胞杆菌菌株3个浓度低温驯化10、15和20 d后,在冷冻条件下松材线虫强毒虫株AMA3、中毒虫株AA3和弱毒虫株YW4的存活率和繁殖量。结果表明:低温驯化15 d和20 d后3株菌株对不同毒力线虫的活力影响较驯化10 d后的更显著。在低温驯化15 d、-20℃冷冻处理1 h后,5×10^(6) CFU/mL浓度菌株GD1处理下,虫株AMA3、AA3和YW4的存活率分别为77.22%、83.68%和84.26%,与对照差异显著;5×10^(5) CFU/mL浓度菌株GD1处理下,虫株AMA3、AA3和YW4的存活率分别为75.76%、80.67%和81.50%,与对照差异显著。5×10^(6) CFU/mL和5×10^(5) CFU/mL浓度菌株GD2处理下,与GD1处理组结果相似,菌株NJSZ-13处理组则与菌株GD1和GD2的结果相反。低温驯化15 d、-20℃冷冻处理1 h后,5×10^(6) CFU/mL浓度菌株GD1处理下,虫株AMA3、AA3和YW4的繁殖量分别为7530、9317和12793条/皿,与对照(3192、3840和5823条/皿)差异显著;5×10^(5) CFU/mL浓度菌株GD1处理时,3个虫株的繁殖量均与对照差异显著。而菌株GD2和NJSZ-13处理后,3个虫株的繁殖量均无显著变化。表明不同芽胞杆菌对松材线虫的低温适应性影响存在差异,松材线虫伴生细菌GD1和马尾松内生细菌GD2能增强其低温适应性,而湿地松内生细菌NJSZ-13菌株则相反。
In order to explore the role of bacteria in the ecological adaptability of pine wood nematode(PWN),Bursaphelenchus xylophilus,the changes of the survival and reproduction of PWNs treated with bacteria,the effects of specific bacteria(nematode-carried bacteria GD1,Pinus massoniana endophyte GD2 and endophyte NJSZ-13 of P.elliottii with nematicidal activity)on the freeze resistance of PWNs with different virulence types(strongly virulent isolate AMA3,normally virulent isolate AA3,weakly virulent isolate YW4)were studied after the three specific Bacillus strains at three different concentrations acclimated for 10,15 and 20 d.The results showed that,in the low-temperature survival test,the effect of bacteria on the survival of nematodes belonging to different virulence types was more significant in the treatment group after 15 days and 20 days of low temperature acclimation than that after ten days of acclimation.The survival rates of AMA3,AA3 and YW4 were 77.22%,83.68%and 84.26%,respectively,which were significantly different from their control groups after 15 days of cryopreservation at-20℃for 1 h with 5×10^(6) CFU/mL strain GD1.Under the treatment with 5×10^(5) CFU/mL strain GD1,the survival rates of AMA3,AA3 and YW4 were 75.76%,80.67%and 81.50%,respectively.The differences were significant when compared with each control group.The results of the strain GD2 treatment with 5×10^(6) CFU/mL and 5×10^(5) CFU/mL were similar to the results in the GD1 treatment group,while the results of the strain NJSZ-13 treatment group were opposite with GD1 and GD2 strains.In the experiment of low temperature reproduction,after 15 days of low-temperature acclimation and freezing treatment at-20℃for 1 h,under the treatment of 5×10^(6) CFU/mL strain GD1,the fecundities of AMA3,AA3 and YW4 were 7530,9317 and 12793 per plate,respectively,which were significantly different from those of their corresponding control groups(3192,3840 and 5823 per plate,respectively).There were significant differences among the three strains and the
作者
严正梅
翟宁宁
谈家金
郝德君
Yan Zhengmei;Zhai Ningning;Tan Jiajin;Hao Dejun(Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2021年第2期442-448,共7页
Journal of Plant Protection
基金
国家重点研发计划(2018YFC1200400)
南京林业大学大学生实践创新训练计划项目(2019NFUSPITP0307)。
关键词
松材线虫
松材线虫伴生细菌
松树内生细菌
低温适应性
Bursaphelenchus xylophilus
PWN-carried bacteria
pine endophyte
low-temperature adaptability
