摘要
A well designed coating for polyetheretherketone(PEEK)implants can provide enough support to overcome crucial medical challenges,which are insufficient osseointegration and high rate of infection.Herein,we utilize the co-deposition of polydopamine(PDA)and copper-citrate nanoclusters to construct a p H-responsive coating on porous PEEK for synergistic bone regeneration,vascular formation and anti-infection.Specifically,this PDA coating released high dose of copper and citrate at lower p H value,which increased intracellular copper content,boosted production of reactive oxygen species and severe damage of protein,leading to killing of 93%planktonic bacterial and eradication of adherent bacteria.At p H of 7.4,the release of copper and citrate were in a slow and sustained behavior,synergistically enhanced vascular formation potential and osteodiffereration of Ad-MSC in vitro.After implanted in rabbit tibia for 6 and 12 weeks,the micro-CT evaluation and histological analysis consistently highlighted the ability of this PDA coating to increase new bone formation adjacent to coated PEEK implant and enhance bone-implant interfacial integration.These results were proven to be related to the synergistic effect that citrate facilitated a 2-fold influx of copper into cells,which not only enhanced the bacteria-killing ability but also encouraged bone regeneration of implants.This present work provides an effective method to control infections while promoting osseointegration simultaneously,which will show tremendous clinical application and can be a solution to current challenges facing orthopedics.
基金
financially supported by the National Key Research and Development Project(2018YFC1106600)
National Natural Science Foundation of China(No.31670974,No.31370954,No.51901003)。
