摘要
目的检验热力及紫外线(ultraviolet,UV)辐照对轮状病毒(rotavirus,RV)的消毒效果,为环境消毒RV提供技术支撑。方法通过计算半数组织培养感染剂量(median tissue culture infective dose,TCID50)检测在热力及紫外线处理后RV的滴度变化,判断消毒效果。结果RV在37℃下24 h内病毒活性无明显变化。在56℃及以上温度下对RV作用10 min即可达到消毒效果。在距离紫外光源15 cm、30 cm处辐射7 min均可对RV产生消毒作用,分别辐射10 min、15 min可达灭活效果。在距离紫外光源1 m处辐射30 min或距离紫外光源2 m处辐射60 min以上均可以达到消毒作用。结论通过56℃及以上温度、近距离UV辐射可对RV进行有效消毒,但距离UV光源较远时则需更长时间的辐射达到消毒作用,为消毒和灭活RV提供了参考方法。
Objective To examine the efficacy of heat and ultraviolet(UV)radiation in disinfecting rotavirus(RV),so as to provide technical basis for environmental disinfection of RV.Methods The disinfection effect on RV was calculated by the method of median tissue culture infective dose(TCID50)using the change of nucleic acid titer after treatment with heat or UV.Results There was no significant change in viral activity of RV at 37℃for 24 h.The disinfection effect could be achieved when RV was treated at 56℃and above for 10 min.Radiation of 7 min at the distance of 15 cm or 30 cm from the UV light source could disinfect the RV,and treatment for 10 min at the 15 cm distance or 15 min at 30 cm could inactivate RV.Disinfection could be achieved by treatment for 30 min at a distance of 1 m from the UV light source or 60 min at a distance of 2 m.Conclusions RV can be effectively disinfected by 56℃or above,or by close to UV radiation.It is recommended to prolong the disinfection time for long distance from the UV light source.This provides a reference method for disinfecting and inactivating RV.
作者
李伯阳
李慧莹
章青
王萌璇
孙晓曼
孔翔羽
王宏
李丹地
庞立丽
裴银辉
Li Boyang;Li Huiying;Zhang Qing;Wang Mengxuan;Sun Xiaoman;Kong Xiangyu;Wang Hong;Li Dandi;Pang Lili;Pei Yinhui(Hebei Key Laboratory for Chronic Diseases,College of Elementary Medicine,North China University of Science and Technology,Tangshan 063000,China;National Health Commission Key Laboratory of Medical Viruses and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《国际病毒学杂志》
2021年第2期108-110,共3页
International Journal of Virology
基金
"十三五"国家科技重大专项课题(2018ZX10734401)
国家自然科学基金(21934005)。
关键词
轮状病毒
物理消毒
紫外线
热力
Rotavirus
Physical disinfection
Ultraviolet ray
Heating
