摘要
在水稻抗倒伏育种中,水稻茎秆纤维素含量作为重要的作物性状表现型数据,用传统方法获取时受人力成本和时间成本的约束,采集群体大小有限。利用高光谱技术能够实现对作物性状信息的快速、无损检测。为探究水稻茎秆纤维素含量近红外光谱反演模型,以田间小区试验的方式,采集水稻灌浆期至成熟期茎秆基部倒2、3节作为实验样本,并在实验室内使用NIRQuest512型号高光谱仪测得茎秆近红外反射光谱数据;采用标准变量正态变换(SNV)、连续小波变换(CWT)及两种方法结合(SNV-CWT)对原始近红外光谱进行预处理,经对比分析,原始光谱经SNV处理后再通过CWT对应6尺度分解最优,然后采用联合区间偏最小二乘法(SiPLS)、迭代保留信息变量法(IRIV)对最优预处理(SNV-CWT)的光谱特征曲线进行光谱特征变量筛选,分别提取了64个和16个特征变量;为优化模型并提高其模型精度,采用IRIV算法对SiPLS所选的特征变量进行二次筛选,得到6个特征变量,特征波长为1200,1207,1325,1470,1482和1492 nm,最后基于优选出的特征变量分别建立水稻茎秆纤维素含量的支持向量机回归(εSVR)和核极限学习机(KELM)预测模型,模型参数(惩罚系数C,核函数系数γ和不敏感参数ε)分别采用灰狼算法(GWO)、差分进化灰狼算法(DEGWO)和自适应差分进化灰狼算法(SaDEGWO)进行优化选择。结果表明,采用SNV-CWT方法光谱预处理后,经SiPLS-IRIV方法筛选的特征变量构建的SaDEGWO优化的SVR模型精度最高,模型参数C,γ,ε分别为302.8382,0.0877,0.0708,测试集的决定性系数(R_(p)^(2))为0.880,均方根误差(RMSE P)为15.22 mg·g^(-1),剩余预测残差(RPD)为2.91,表明模型具有较好的预测能力,可为水稻茎秆纤维素含量预测提供参考。
In lodging resistance breeding of rice,the cellulose content of rice stem,as an important phenotypic data of crop traits,is constrained by human and time costs,which makes the size of the collecting population limited.The rapid and non-destructive detection of crop traits information can be achieved by using hyperspectral technology.In lodging resistance breeding of rice,rice stem cellulose content is one of the important character information.In order to explore the near-infrared spectral inversion model of cellulose content in rice stem,the bottom 2 and 3 segments of rice stem base formfilling stage to maturity stage were collected as experimental samples by field plot experiment,and the stem near-infrared reflectance spectrum data were measured by NIRQuest512 hyper-spectrometer in the laboratory.Standard normal variate(SNV),continuous wavelet transform(CWT),and the combination of the two methods(SNV-CWT)were used to pretreat the original near-infrared reflectance spectrum.Through comparative analysis,it was found that the original spectrum was optimized when it is firstly processed by SNV and then decomposed by CWT at 6 scales,and then the spectral characteristic variables are screened by the synergy interval PLS(SiPLS)method and iteratively retaining informative variables(IRIV)method for the characteristic spectral curve obtained by the optimal pretreatment(SNV-CWT).64 and 16 characteristic variables were extracted,respectively.To optimize the model and improve its accuracy,the IRIV algorithm was used to conduct secondary screening of the characteristic variables selected by SiPLS,and 6 characteristic variables were obtained with the characteristic wavelengths of 1200,1207,1325,1470,1482 and 1492 nm.Finally,the support vector machine regression(ε-support vector machine regression,εSVR)and the kernel-based extreme learning machine(KELM)prediction model were established based on the selected characteristic variables.The model parameters(penalty coefficient C,kernel function coefficientγand insensitive parame
作者
徐博
许童羽
于丰华
张国圣
冯帅
郭忠辉
周长献
XU Bo;XU Tong-yu;YU Feng-hua;ZHANG Guo-sheng;FENG Shuai;GUO Zhong-hui;ZHOU Chang-xian(College of Information and Electrical Engineering,Shenyang Agricultural University,Shenyang 110866,China;Liaoning Agricultural Information Engineering Technology Research Center,Shenyang 110866,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2021年第6期1775-1781,共7页
Spectroscopy and Spectral Analysis
基金
国家“十三五”重点研发计划(2016YFD0200600)
辽宁省教育厅青年科技人才“育苗”项目(LSNQN201903)资助。
关键词
水稻茎秆
纤维素
近红外光谱
光谱预处理
光谱特征变量
反演模型
Rice stem
Cellulose
Near-infrared spectroscopy
Spectral pretreatment
Spectral characteristic variables
Inversion model
