摘要
目的探讨剪切波弹性成像(SWE)评估不同大小人源性裸鼠三阴性乳腺癌弹性特征与临床病理特征的关系。方法选择人乳腺癌MDA-MB-231细胞株,采用细胞悬液注射法对30只雌性BALB/c裸鼠建立人源性三阴性乳腺癌模型,其中27只裸鼠成瘤。选择其中行超声和SWE检查的21只裸鼠21个瘤体作为研究对象。根据移植瘤大小分为三组,即A组(≤5 mm)、B组(>5,≤10 mm)、C组(>10,≤15 mm)。超声测量移植瘤最大径并选择其最大切面行SWE,获得移植瘤的弹性参数,即最大弹性值(Emax)、平均弹性值(Emean)、弹性值标准差(Esd)。对照病理结果,分析移植瘤的Ki67与弹性参数的关系。结果随着移植瘤增大,Emax、Esd、Ki67增加(均P<0.05)。病灶最大径、Emax、Esd、Ki67在B组(P<0.001,P=0.006,P=0.002,P=0.026)和C组(P<0.001,P<0.001,P<0.001,P=0.028)显著大于A组,余参数在各组间差异无统计学意义(均P>0.05)。移植瘤大小与Emax(r_(s)=0.673,P=0.001)、Esd(r_(s)=0.661,P=0.001)、Ki67(r_(s)=0.509,P=0.018)呈显著正相关。结论SWE的Emax和Esd可反映三阴性乳腺癌病灶生长过程中的硬度变化和生物学行为。
Objective To investigate the stiffness characteristic of triple-negative human breast cancer at different size in a implantation nude female mice model using shear wave elastography(SWE)and to compare the clinical pathologic features of tumors with elasticity variables.Methods Human breast cancer MDA-MB-231 cells were injected into 30 nude female mice and 27 transplanted tumors were successfully found in nude female mice.Ultrasound and SWE were longitudinally performed on maximum diameter plane of 21 tumours in 21 nude mice.The elastic parameters of maximal elasticity(Emax),mean elasticity(Emean)and standard deviation of elasticity(Esd)were recorded.The mice were divided into 3 groups according to the tumor size.They were group A with tumor size less than or equal to 5 mm,group B with tumor size greater than 5 mm and less than or equal to 10 mm,group C with tumor size larger than 10 mm and smaller than or equal to 15 mm.Compared with pathology,the relationships between Ki67 of transplanted tumor and elastic parameters were analyzed.Results As the transplanted tumors increased,the values of Emax,Esd,Ki67 all increased.The lesions maximal size,Emax,Esd,Ki67 were significant higher in group B(P<0.001,P=0.006,P=0.002,P=0.026)and group C(P<0.001,P<0.001,P<0.001,P=0.028)than group A.The other parameters were not significantly different among the groups(all P>0.05).The size of transplanted tumors was significantly and positively correlated with Emax(r_(s)=0.673,P=0.001),Esd(r_(s)=0.661,P=0.001),and Ki67(r_(s)=0.509,P=0.018).Conclusions SWE Emax and Esd can reflect the tumor tissue stiffness change and biological activity during the tumor growth.
作者
智文祥
周瑾
刘晁旭
周世崇
常才
Zhi Wenxiang;Zhou Jin;Liu Chaoxu;Zhou Shichong;Chang Cai(Department of Ultrasonography,Fudan University Shanghai Cancer Center&Department of Oncology,Shanghai Medical College,Fudan University,Shanghai 200032,China)
出处
《中华超声影像学杂志》
CSCD
北大核心
2021年第4期346-350,共5页
Chinese Journal of Ultrasonography
基金
国家自然科学基金青年项目(81801701)
国家自然科学基金重点项目(81830058)。
关键词
超声检查
剪切波弹性成像
裸鼠
三阴性乳腺癌
Ultrasonography
Shear wave elastography
Nude
Triple-negative breast cancer
