摘要
软腐病是甘薯贮藏期最具破坏力的病害之一,病原菌是匍枝根霉(Rhizopus stolonifer),匍枝根霉从伤口侵染,利用伤口的营养物质进行繁殖,破坏细胞壁,造成薯块软烂。本研究以不同时期收获的6个甘薯品种块根为试验材料,通过薯片接菌碟法鉴定软腐病抗性,测定薯块的质构特性(硬度、黏附性、黏附力、内聚性、弹性、咀嚼性、胶黏性)、营养物质(干率、淀粉、果糖、葡萄糖、蔗糖、粗蛋白、粗纤维)、抗性酶活(POD、PPO、PAL)等生理指标,对各指标值进行相关分析、灰色关联度分析、隶属函数分析,筛选和综合评价软腐病抗性指标。根据病斑直径将甘薯软腐病抗性划分等级,通过病斑直径和各指标值的相关分析、灰色关联度分析,筛选抗软腐病指标并确定其权重,再进行隶属函数分析,得到不同材料软腐病抗性综合评价值(D值),通过综合评价值和病斑直径比较及相关分析验证指标筛选的可靠性。试验材料的软腐病抗性结果显示,90 d收获甘薯软腐病抗性表现为抗病,105 d收获甘薯整体表现为感病和高感,120、135、150 d收获甘薯软腐病整体抗性为中抗,同时筛选出果糖含量、咀嚼性、内聚性、弹性、粗蛋白含量、POD、PAL酶活性等7个可评价甘薯软腐病抗性的指标。本研究可为甘薯抗软腐新品种选育提供种质并为甘薯软腐病抗性评价及抗软腐机制研究提供理论依据。
Soft rot is one of the most destructive diseases during sweetpotato storage.Cell walls were destroyed and soft rot was caused by Rhizopus Stolonifer,which invaded from wounds and propagated with nutrients from wounds.Six varieties of sweetpotato roots in different harvest period were used as the experimental material to identify the resistance index to soft rot,through the inoculation with sweetpotato chips.Physiological indexes including texture of roots(hardness,adhesion,adhesion force,cohesiveness,elasticity,chewiness,and glue viscosity),nutrients(dry matter content,starch,fructose,glucose,sucrose,crude protein and crude fiber),resistance enzyme activity(POD,PPO,PAL)were investigated in this study.Their correlation analysis,grey correlation analysis of each index and subordinate function analysis were applied in screening and comprehensive evaluation of soft rot resistance.The soft rot resistance of sweetpotato roots was graded based on the disease spot diameter.The index and weight of soft rot resistance were conducted by correlation analysis and grey correlation analysis of disease spot diameter and index values.Comprehensive evaluation value(D-value)of six varieties of sweetpotato roots at different harvest stages were calculated using the membership function analysis.The reliability of indicators selection was verified through correlation analysis of D-value based on the disease spot diameter.Soft rot resistance of sweetpotato was high in 90 day at harvest stage,moderate resistance in 120,135,150 day of harvest stage,and hypersensitivity and susceptibility in 105 day of harvest stage.Seven indexes,including fructose content selection,chewiness,cohesiveness,elasticity,protein content,POD and PAL enzyme activity,were filtered out to estimate the resistance of soft rot in sweetpotato.These results could provide the germplasm information for selection and breeding of new sweetpotato varieties resistant to soft rot and could serve as a basis for subsequent assessment of sweetpotato resistant to soft rot and the
作者
张思梦
倪文荣
吕尊富
林燕
林力卓
钟子毓
崔鹏
陆国权
ZHANG Si-Meng;NI Wen-Rong;LYU Zun-Fu;LIN Yan;LIN Li-Zhuo;ZHONG Zi-Yu;CUI Peng;LU Guo-Quan(College of Agriculture and Food Science,Zhejiang Agriculture and Forest University/Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,Hangzhou 311300,Zhejiang,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第8期1450-1459,共10页
Acta Agronomica Sinica
基金
国家现代农业产业技术体系建设专项(CARS-10-B19)
国家自然科学基金项目(31671750)
浙江省农业(粮食)新品种选育重大科技专项子专题(2016C02050-7-5)资助。
关键词
甘薯
软腐病抗性
收获期
鉴定指标
sweetpotato
soft rot resistance
harvest stage
appraisal indicators
