摘要
目的对用于骨组织工程的体内生物反应器(in vivo bioreactor,IVB)的研究进展进行综述,为后续研究提供参考。方法查阅近年来骨组织工程IVB相关文献,从IVB构建原则、构建IVB的组织类型、部位及方法,以及IVB用于构建组织工程骨的优劣等方面进行综述。结果 IVB是利用机体自我再生能力,把身体作为生物反应器在损伤部位或能够支持新组织再生的异位部位再生新组织或器官。IVB可由组织瓣(皮下袋、肌瓣/袋、筋膜瓣、骨膜瓣、大网膜瓣/腹腔)和轴向血管蒂(轴向血管束、动静脉环)单独或联合构建。使用IVB能预构与骨缺损形状、大小匹配的血管化组织工程骨。预构的骨组织可作为游离骨移植物、带蒂骨瓣或游离骨瓣修复骨缺损。IVB解决了传统骨组织工程血管化不足的问题。结论 IVB是一种有临床应用前景的血管化组织工程骨预构和骨缺损修复重建的方法,在修复大型复杂骨缺损方面有着独特优势。但因IVB存在构建复杂以及手术并发症等问题影响了其临床应用,后续需致力于开发一种简单、安全、高效的IVB。
Objective To review the research progress of in vivo bioreactor(IVB) for bone tissue engineering in order to provide reference for its future research direction. Methods The literature related to IVB used in bone tissue engineering in recent years was reviewed, and the principles of IVB construction, tissue types, sites, and methods of IVB construction, as well as the advantages of IVB used in bone tissue engineering were summarized. Results IVB takes advantage of the body’s ability to regenerate itself, using the body as a bioreactor to regenerate new tissues or organs at injured sites or at ectopic sites that can support the regeneration of new tissues. IVB can be constructed by tissue flap(subcutaneous pocket, muscle flap/pocket, fascia flap, periosteum flap, omentum flap/abdominal cavity) and axial vascular pedicle(axial vascular bundle, arteriovenous loop) alone or jointly. IVB is used to prefabricate vascularized tissue engineered bone that matched the shape and size of the defect. The prefabricated vascularized tissue engineered bone can be used as bone graft, pedicled bone flap, or free bone flap to repair bone defect. IVB solves the problem of insufficient vascularization in traditional bone tissue engineering to a certain extent. Conclusion IVB is a promising method for vascularized tissue engineered bone prefabrication and subsequent bone defect reconstruction, with unique advantages in the repair of large complex bone defects. However, the complexity of IVB construction and surgical complications hinder the clinical application of IVB. Researchers should aim to develop a simple, safe, and efficient IVB.
作者
王建
王晓
甄平
樊博
WANG Jian;WANG Xiao;ZHEN Ping;FAN Bo(First School of Clinical Medicine,Gansu University of Chinese Medicine,Lanzhou Gansu,730000,P.R.China;Orthopaedic Center,the 940th Hospital of PLA Joint Logistics Support Force,Lanzhou Gansu,730000,P.R.China;School of Design and Art,Lanzhou University of Technology,Lanzhou Gansu,730000,P.R.China)
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2021年第5期627-635,共9页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金青年基金资助项目(31800812)
军队医学科技青年培育计划孵化项目(20QNPY069)
甘肃省青年科技基金(20JR5RA593)。
关键词
体内生物反应器
骨组织工程
血管化
预构
In vivo bioreactor
bone tissue engineering
vascularization
prefabrication
