摘要
水体浮游真菌是水生生态系统中重要组成部分。为探明水库水体真菌群落结构的变化特征及其与环境因子的相关关系,该文运用高通量DNA测序技术诊断水库水体真菌群落结构的异质性,并结合主成分分析方法研究真菌群落与各水质参数的关系。结果表明,受酸性矿山废水(AMD)影响的水库水体EC、DO、SO_(4)^(2-)及重金属值较高,而pH和DOC含量较低。pH变化范围在4.78~6.13,SO_(4)^(2-)浓度范围在384.13~718.66 mg/L,EC变化范围在399.00~1177.00μS/cm。水体真菌群落高通量DNA测序共发现2073个分类操作单元(OTUs),隶属8个门20个纲240个属,各水库水体优势菌门主要有担子菌门(Basidiomycota)、子囊菌门(Ascomycota)和壶菌门(Chytridiomycota),毛霉菌门(Mucoromycota)和顶复亚门(Apicomplexa)是未受酸性矿山废水影响水库水体中除担子菌门、子囊菌门和壶菌门外的2个优势门类。主成分分析表明Ascomycota、Basidiomycota和Chytridiomycota受DO正向调控,而与pH、EC、NH_(4)^(+)-N、DOC、SO42-及各重金属离子(Pb^(2+)、Mn^(2+)和Ni^(2+))呈负相关关系。DOC、水温、pH、总磷(TP)正向调控浮游真菌Mucoromycota和Apicomplexa,其中与DOC正相关性在p<0.01水平,与水温在p<0.05水平,EC、Eh、DO、NH_(4)^(+)-N、总氮(TN)及各重金属离子(Pb^(2+)、Mn^(2+)和Ni^(2+))与Mucoromycota和Apicomplexa呈负相关关系,其中与TN达p<0.05水平。由此推测受AMD影响与未受AMD影响水库水体浮游真菌群落与水质参数关系存在异同之处。该研究结果为受AMD影响下水库水质参数与真菌群落结构的偶联机制提供科学依据。
Phytoplankton is an important part of aquatic ecosystem.To explore the change characteristics of fungal community structure and its correlation with environmental factors,the heterogeneity of fungal community structure in reservoir water was diagnosed by high-throughput DNA sequencing,and the relationship between fungal community and water quality parameters was studied by principal component analysis(PCA).The results showed that the values of EC,DO,heavy metal and SO_(4)^(2-)were higher,while the contents of pH and DOC were lower in the reservoir affected by AMD.The p H range was 4.78~6.13,the SO_(4)^(2-)concentration range was 384.13~718.66 mg/L,and the EC range was 399.00~1177.00μS/cm.A total of 2073 OTUs,belonging to 8 phylum,20 class and 240 genera,were found by high-throughput DNA sequencing of water fungal community.The dominant fungi in the reservoirs mainly include Basidiomycota,Ascomycota and Chytridiomycota.Mucoromycota and Apicomplexa are the two dominant phylum in the water of the reservoir unaffected by AMD,except Basidiomycota,Ascomycota and Chytridiomycota.Principal component analysis showed that Ascomycota,Basidiomycota and Chytridiomycota were positively regulated by DO and negatively correlated with pH,EC,NH_(4)^(+)-N,DOC,SO_(4)^(2-)and various heavy metal ions(Pb^(2+),Mn^(2+)and Ni^(2+)).DOC,T,pH and TP positively regulated the Mucoromycota and Apicomplexa of the phytofungi,and the positive correlation with DOC was at p<0.01 level,while that with T was at p<0.05 level.EC,Eh,DO,NH_(4)^(+)-N,TN and heavy metal ions(Pb^(2+),Mn^(2+)and Ni^(2+))were negatively correlated with Mucoromycota and Apicomplexa,and the correlation with TN was at p<0.05 level.It is speculated that there are similarities and differences in the relationship between water quality parameters and planktonic fungi in the reservoir affected by AMD and not affected by AMD.The results provide scientific basis for the coupling mechanism between water quality parameters and fungal community structure of reservoir under the influence
作者
刘闪
曹星星
吴攀
廖路
廖家豪
LIU Shan;CAO Xingxing;WU Pan;LIAO Lu;LIAO Jiahao(College of Resources and Environmental Engineering,Guiyang 550025,China;Key Laboratory of Karst Geological Resources and Environment of Ministry of Education,Guizhou University,Guiyang 550025,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2021年第2期1-8,共8页
Environmental Science & Technology
基金
国家自然科学基金(U1612442)
贵州省科技计划项目(黔科合平台人才[2016]5664,黔科合[2016]支撑2835)
贵州省人才基地项目(RCJD2018-21)。
关键词
酸性矿山排水
水库
真菌群落
高通量测序
环境因子
acid mine drainage(AMD)
reservoir
fungal community
high throughput sequencing
environmental factors
