摘要
目的:研究柚皮苷(NRG)与胃蛋白酶(Pep)的关系,为从分子水平上研究柚皮苷与Pep的作用特点提供信息。方法:配制柠檬酸-磷酸盐缓冲液,溶解并定容Pep,精确称取NRG加入乙醇溶解定容,4℃保存备用。采用紫外吸收光谱、荧光光谱、同步荧光光谱法,通过计算相关参数来判断NRG和Pep相互作用特点。结果:NRG与Pep的结合是自由能降低的分子作用过程。NRG对Pep的猝灭机制为动态猝灭。NRG可以自发地与Pep相结合。随着NRG浓度增大,Pep同步荧光强度逐渐降低。NRG和Pep之间有相互作用。结论:NRG与Pep的相互作用机制可由荧光光谱的变化推断得出,NRG与Pep产生了相互作用或自发结合,Pep的荧光猝灭可能是形成新的复合物引起的复合式静态猝灭,大约1个NRG分子与1个Pep分子结合形成配合物并伴随能量转移。
Objective:To study the relationship between naringin(NRG)and pepsin(Pep),so as to provide information for studying the interaction characteristics of NRG and Pep at molecular level.Methods:Pep was diluted to constant volume by the prepared citric acid-phosphate buffer solution,NRG was accurately weighed and diluted to constant volume by adding ethanol,and stored at 4℃.UV absorption spectrum,fluorescence spectrum and synchronous fluorescence spectrum were used to determine the interaction characteristics of NRG and Pep by calculating the relevant parameters.Results:The binding of NRG to Pep was a molecular process with reduced free energy.The quenching mechanism of PEP by NRG was dynamic.NRG could spontaneously combine with Pep.With the increase concentration of NRG,the synchronous fluorescence intensity of Pep decreased gradually.There was an interaction between NRG and Pep.Conclusion:The interaction mechanism of NRG and Pep can be inferred from the change of fluorescence spectra.The interaction or spontaneous binding between NRG and PEP can be inferred.The fluorescence quenching of Pep may be a compound static quenching caused by the formation of a new complex.About one NRG molecule combines with a Pep molecule to form a complex with energy transfer.
作者
杨鑫
徐晶
马坤
王娜
杨广德
南冠军
YANG Xin;XU Jing;MA Kun;WANG Na;YANG Guangde;NAN Guanjun(The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China;Shaanxi Provincial People's Hospital, Xi'an 710068, China;School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China)
出处
《中医药信息》
2021年第5期11-16,共6页
Information on Traditional Chinese Medicine
基金
国家自然科学基金项目(81373368)。
