摘要
目的研究儿童穿孔性阑尾炎患者外周血单核细胞表面HLA-DR/CD14表达率及与临床诊断治疗的相关性。方法选择西安市儿童医院2016年1月~2017年5月收治的49例经传统开腹阑尾切除术治疗的穿孔性阑尾炎患儿为开腹术组,选择同期该院经腹腔镜阑尾切除术治疗的55例穿孔性阑尾炎患儿为腹腔镜组,另选取同期该院健康体检25例患儿为对照组。应用流式细胞术检测法检测外周血HLA-DR/CD14的表达率。结果术前开腹术组和腹腔镜组的外周血HLA-DR/CD14表达率均低于对照组,其差异具有统计学意义(F=50.231,P=0.002),术后2天和5天,开腹术组和腹腔镜组的外周血HLA-DR/CD14表达率较术前均显著升高,其差异具有统计学意义(t=6.383,9.509,5.150,11.127,均P<0.05),腹腔镜组的外周血HLA-DR/CD14表达率高于开腹术组,其差异具有统计学意义(t=3.235,2.215,均P<0.05)。相较于开腹术组,腹腔镜组外周血单核细胞表面HLA-DR/CD14表达率变化的幅度较大,其差异具有统计学意义(t=40.137,34.566,69.325,均P<0.001)。结论腹腔镜阑尾切除术患儿外周血单核细胞表面HLA-DR/CD14表达率较高,能有效增强患儿免疫功能,对康复产生积极作用。
Objective To study the expression rate of HLA-DR/CD14 on peripheral blood monocytes in children with perforated appendicitis and its correlation with clinical diagnosis and treatment.Methods Choose Xi’an at children’s Hospital between January 2016 and May 2017,49 cases treated by traditional open appendectomy in treatment of perforated appendicitis patients for open surgery group,choose the same hospital laparoscopic appendectomy in treatment of 55 cases of perforated appendicitis patients for laparoscopic group,the other selected at the same time the hospital physical examination 25 cases as control group.Flow cytometry was used to detect the expression rate of HLA-DR/CD14 in peripheral blood.Results Preoperative laparotomy surgery group and the laparoscopic group in the peripheral blood of HLA-DR/CD14 expression rate were lower than the control group,the difference was statistically significant(F=50.231,P=0.002),2 and 5 days postoperatively,open surgery and laparoscopic group HLA-DR/CD14 expression rate of peripheral blood from preoperative were significantly higher,the difference was statistically significant(t=6.383,9.509,5.150,11.127,all P<0.05),the peripheral blood of laparoscopic group HLA-DR/CD14 expression rate higher than the open surgery group,the difference was statistically significant(t=3.235,2.215,all P<0.05).Compared with the laparotomy group,the expression rate of HLA-DR/CD14 on the surface of peripheral blood monocytes in the laparoscopic group showed a larger change,and the difference was statistically significant(t=40.137,34.566,69.325,all P<0.001).Conclusion The expression rate of HLA-DR/CD14 on peripheral blood monocyte surface of children with laparoscopic appendectomy is relatively high,which can effectively enhance the immune function of children and play a positive role in rehabilitation.
作者
刘涛
李站虎
LIU Tao;LI Zhan-hu(Xi’an Children’s Hospital,Xi’an 710003,China)
出处
《现代检验医学杂志》
CAS
2021年第3期141-143,共3页
Journal of Modern Laboratory Medicine
