摘要
目的探讨染色质重塑基因-AT富集作用域2基因(AIRD2)在乳腺Paget病(MPD)发生发展中的作用和临床意义。方法选取2011年3月至2019年1月徐州医科大学附属医院收治的30例MPD伴发乳腺癌患者,对其MPD石蜡包埋组织标本及伴发的乳腺癌石蜡包埋组织标本共30对,采用免疫组织化学法检测ARID2及乳腺癌分子标志物[雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(HER2)和肿瘤细胞增殖核抗原(Ki-67)]的表达情况,并采用免疫荧光原位杂交(FISH)检测HER2的表达。分析配对样本间ARID2的表达差异,及ARID2表达与乳腺癌分子标志物、分子分型(根据ER、PR、HER2结果进行分型)、临床病理参数、生存时间的关系,同时分析配对样本间分子表型的一致性。结果ARID2在MPD中阳性率为66.7%,在伴发的乳腺癌组织中阳性率为30.0%,差异有统计学意义(P<0.01)。ARID2的表达与MPD中ER、PR、HER2、Ki-67的表达、伴发乳腺癌类型及分子分型无明显关联性(P均>0.05),与淋巴结转移、分期及生存时间亦无明显关联性(P均>0.05)。ER、PR、HER2的表达和分子分型在MPD表皮内瘤细胞与伴发乳腺癌两种组织中的一致率分别为70.0%、73.3%、96.7%和63.3%,两种组织的表达一致率以HER2最高。结论MPD表皮内病变与伴发的乳腺癌关系密切,ARID2在MPD的发生发展中起作用,尤其HER2可能在MPD的发生发展中起重要作用,但其表达的意义和机制尚待进一步研究。
Objective To explore the role and clinical significance of chromatin remodeling gene-AT-rich interactive domain 2(AIRD2)in the occurrence and development of mammary Paget′s disease(MPD).Methods Thirty MPD patients complicated with breast cancer admitted to the Affiliated Hospital of Xuzhou Medical University from March 2011 to January 2019 were selected.Thirty pairs of paraffin-embedded tissue samples from patients with MPD and associated breast cancer were collected.The expression levels of ARID2 and breast cancer molecular markers[estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor 2(HER2),tumor cell proliferating nuclear antigen(Ki-67)]were detected by immunohistochemistry,and HER2 expression was detected by immunofluorescence in situ hybridization(FISH)in two types of tissues.The difference of ARID2 expression between paired samples,the associations of ARID2 expression with breast cancer molecular markers,molecular typing(based on ER,PR,HER2 results),clinicopathological parameters and survival were analyzed,and the consistency of molecular phenotype among paired samples was analyzed.Results The positive rate of ARID2 was 66.7%in MPD tissues and 30.0%in breast cancer tissues(P<0.01).ARID2 expression was not significantly correlated with expressions of ER,PR,HER2,Ki67,associated breast cancer type,molecular typing,lymph node metastasis,clinical stage and survival(all P>0.05).The consistency rates of expressions of ER,PR,HER2 and molecular typing between MPD epidermal tumor cells and associated breast cancer tissues were 70.0%,73.3%,96.7%and 63.3%,respectively.HER2 had the highest consistency rate between two types of tissues.Conclusions MPD is closely related to associated breast cancer.ARID2 and especially,HER2 may play the role in the occurrence and development of MPD.However,the further study is needed for the significance and mechanism of ARID2 and HER2 expressions.
作者
胡曦文
向臣希
马东慎
刘慧
HU Xi-wen;XIANG Chen-xi;MA Dong-shen;LIU Hui(Department of Pathology,Xuzhou Medical University,Xuzhou,Jiangsu 221002,China;不详)
出处
《中国临床研究》
CAS
2021年第4期460-465,共6页
Chinese Journal of Clinical Research
