摘要
Theranostic nanoprobes can potentially integrate imaging and therapeutic capabilities into a single platform,offering a new personalized cancer diagnostic tool.However,there is a growing concern that their clinical application is not safe,particularly due to metal-containing elements,such as the gadolinium used in magnetic resonance imaging(MRI).We demonstrate for the first time that the photothermal melting of the DNA duplex helix was a reliable and versatile strategy that enables the on-demand degradation of the gadolinium-containing MRI reporter gene from polydopamine(PDA)-based theranostic nanoprobes.The combination of chemotherapy(doxorubicin)and photothermal therapy,which leads to the enhanced anti-tumor effect.In vivo MRI tracking reveals that renal filtration was able to rapidly clear the free gadolinium-containing MRI reporter from the mice body.This results in a decrease in the long-term toxic effect of theranostic MRI nanoprobes.Our findings may pave the way to address toxicity issues of the theranostic nanoprobes.
诊疗纳米探针可将成像和治疗功能整合到同一平台中,为个性化癌症诊疗提供了一种新的工具.目前磁共振成像(MRI)诊疗纳米探针多含有重金属元素钆,其临床应用的安全性越来越受到了人们的关注.本文设计了一种基于双链DNA熔融机制的光热诊疗纳米探针,能够从聚多巴胺基纳米探针上按需降解含钆的MRI分子基团.体内MRI显示降解的含钆分子基团能够通过肾脏从小鼠体内快速清除,从而降低了该诊疗纳米探针的长期毒性.本研究为诊疗纳米探针的毒性问题提供了可借鉴的解决方案.
作者
Wenbo Sun
Zonghua Wang
Jianhua Liu
Chunhuan Jiang
Weihua Chen
Bin Yu
Wei Wang
Lehui Lu
孙文博;王宗花;刘建华;姜春环;陈伟华;于滨;王唯;逯乐慧(College of Materials Science and Engineering,College of Chemistry and Chemical Engineering,Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials,Instrumental Analysis Center of Qingdao University,Qingdao University,Qingdao 266071,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;Department of Radiology,Second Hospital of Jilin University,Changchun 130041,China)
基金
supported by the National Natural Science Foundation of China(21635007 and 21605137)
the National Key Research and Development Program of China(2016YFA0203200)
Natural Science Foundation of Shandong Province(2018GGX102030)
Taishan Scholar Program of Shandong Province(ts201511027)
K.C.Wong Education Foundation。
