摘要
目的:研究射干种子的质量检测规范并建立射干种子质量等级标准。方法:对不同产地射干种子进行净度分析,直径分类后测定不同直径大小的射干种子的千粒重、含水率、生活力和发芽率。利用K均值聚类算法(K-means)进行聚类分析,初步制定射干种子的质量分级标准。结果:射干种子大小存在明显的地域性差异,湖北孝昌、大悟及黄冈农科院射干种子粒径较大,直径多在4.5~5.5 mm;湖北团风、云南曲靖、河北安国的射干种子较之其他3个产地的射干种子粒径较小,种子直径多在3.5~5.0 mm。射干种子采用10%NaClO去除假种皮可有效打破种子休眠,显著提高种子发芽率。在此基础上,以种子直径大小为主要分级指标,种子含水率、生活力、发芽率、净度作为重要参考指标,初步将射干种子质量分为3个等级,一级:种子直径≥5.5 mm,含水率≤10%,生活力≥94%,发芽率≥60%,净度≥93%;二级:种子直径≥4.5 mm,含水率≤10%,生活力≥90%,发芽率≥55%,净度≥85%;三级:种子直径≥3.5 mm,含水率≤10%,生活力≥84%,发芽率≥45%,净度≥80%。结论:该研究初步制定了射干种子质量分级标准,为射干种子的质量评价和良种选育提供参考依据。另外,射干种子成熟度和种子存放时间对种子质量影响较大,生产上宜选择完全成熟(乌黑色)种子和新种子。
Objective: To study the quality inspection standard of seeds of Belamcanda chinensis and establish the quality grading standard of seeds of B. chinensis. Method:The purity of seeds of B. chinensis from different producing areas was analyzed,and the 1 000-grain weight,water content,viability and germination rate of seeds of B. chinensis with different diameters were determined after diameter classification. K-means was used for cluster analysis to preliminarily formulate the quality grading standard of seeds of B. chinensis. Result:There were obvious regional differences in the size of seeds of B. chinensis. The particle size of seeds of B. chinensis of Xiaochang,Dawu and Huanggang Academy of Agricultural Sciences in Hubei Province was larger and mostly between 4.5 mm to 5.5 mm. The seeds of B. chinensis from Tuanfeng,Qujing,Yunnan,and Anguo,Hebei province had smaller particle sizes than those from the other three producing areas,which were mostly between 3.5 mm to 5.0 mm. Arils in seeds of B. chinensis were removed by 10% NaClO to effectively break seed dormancy and significantly improve the seed germination rate. On this basis,the seed diameter was taken as the main grading index,and the seed moisture content,viability,germination rate and purity were taken as important reference indexes. The quality of seeds of B. chinensis was preliminarily divided into three grades,grade Ⅰ :seed diameter≥5.5 mm,moisture content≤10%,viability≥94%,germination rate≥60%,and cleanliness≥93%;grade Ⅱ :seed diameter≥4.5 mm,moisture content≤10%,viability≥90%,germination rate≥55%,and cleanliness≥85%;and grade Ⅲ :seed diameter≥3.5 mm,moisture content≤10%,viability≥84%,germination rate≥45%,cleanliness≥80%. Conclusion: In this study,the quality grading standard of seeds of B. chinensis was preliminarily established to provide reference for the quality evaluation of seeds of B. chinensis and the breeding of improved varieties. In addition,the maturity and the storage time of seeds of B. chinensis have a
作者
刘雨
杨雅雯
朱芸芸
刘启
黄必胜
刘大会
LIU Yu;YANG Ya-wen;ZHU Yun-yun;LIU Qi;HUANG Bi-sheng;LIU Da-hui(Hubei Provincial Key Laboratory of Traditional Chinese Medicine(TCM)Resources and TCM Chemistry,Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2021年第10期109-116,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点研发计划项目(2017YFC1700704)
现代农业产业技术体系建设专项(CARS-21)。
关键词
射干
种子
发芽率
质量检测
质量分级
Belamcanda chinensis
seed
germination percentage
quality inspection
quality classification