摘要
目的分析HNF-4α基因多态性与非酒精性脂肪性肝病(NAFLD)的关系。方法选择100例NAFLD患者作为观察组,并根据患者病变程度分为轻度组(28例)、中度组(36例)、重度组(36例)3个亚组。选择同时期查体的100例健康人作为对照组。比较2组一般临床资料及血生化指标等,采集2组外周静脉血,应用聚合酶链式反应(PCR)检测HNF-4α基因rs4812829位点的多态性;Logistic回归分析NAFLD的影响因素。结果2组的性别、年龄差异无统计学意义(P>0.05)。观察组收缩压(SBP)、舒张压(DBP)、体质量指数(BMI)、腰臀比(WHR)均明显高于对照组(P<0.05)。对照组、轻度组、中度组、重度组丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、谷氨酰转肽酶(γ-GT)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、葡萄糖(GLU)、胰岛素抵抗指数(HOMA-IR)及尿酸(UA)水平大体呈升高趋势,高密度脂蛋白胆固醇(HDL-C)水平呈下降趋势(P<0.05)。观察组与对照组HNF-4αrs4812829等位基因及基因型分布差异无统计学意义(P>0.05);观察组不同亚组间HNF-4αrs4812829等位基因及基因型分布差异无统计学意义(P>0.05);观察组HNF-4αrs4812829位点不同基因型携带者之间ALT、AST、γ-GT、TC、TG、HDL-C、LDL-C、GLU、HOMA-IR及UA水平差异无统计学意义(P>0.05);基因型为AG、AA的个体发生NAFLD的风险与基因型为GG个体相比均未增加。结论HNF-4α基因rs4812829位点多态性及等位基因的分布与NAFLD的发生无明显相关。
Objective To analyze the relationship between HNF-4αgene polymorphism and non-alcoholic fatty liver disease(NAFLD).Methods One hundred patients with NAFLD were selected as the observation group,and patients were divided into three subgroups according to the degree of disease:mild group(28 cases),moderate group(36 cases)and severe group(36 cases).One hundred healthy people for physical examination during the same period were selected as the control group.The general clinical data and blood biochemical indexes were compared between the two groups.The peripheral venous blood samples were collected from the two groups of patients,and the HNF-4αgene loci rs4812829 polymorphism was detected by polymerase chain reaction(PCR).The influencing factors of NAFLD was analyzed by Logistic regression analysis.Results There were no significant differences in gender and age between the two groups(P>0.05).The systolic blood pressure(SBP),diastolic blood pressure(DBP),body mass index(BMI),waist-to-hip ratio(WHR)were significantly higher in the observation group than those of the control group(P<0.05).Alanine aminotransferase(ALT),aspartate aminotransferase(AST),glutamyl transpeptidase(γ-GT),triglyceride(TG),total cholesterol(TC),low density liptein cholesterol(LDL-C),glucose(GLU),insulin resistance index(HOMA-IR)and uric acid(UA)levels generally showed an upward trend in the control group,mild group,moderate group and severe group(P<0.05),while the high density lipoprotein cholesterol(HDL-C)level showed a downward trend(P<0.05).There were no statistically significant differences in the distribution of HNF-4αrs4812829 allele and genotype between the observation group and the control group(P>0.05).There were no statistically significant difference in the distribution of HNF-4αrs4812829 allele and genotype between different subgroups in the observation group(P>0.05).There were no statistically significant differences in ALT,AST,γ-GT,TC,TG,HDL-C,LDL-C,GLU,HOMA-IR and UA levels between different genotype carriers at rs4812829 of H
作者
黄海燕
李毅
吕其军
周芳
王济红
杨长春
徐娟
HUANG Hai-yan;LI Yi;LYU Qi-jun;ZHOU Fang;WANG Ji-hong;YANG Chang-chun;XU Juan(Department of Infectious Diseases,Central Hospital of Shengli Oilfield,Dongying 257100,China;Department of Clinical Laboratory,Central Hospital of Shengli Oilfield,Dongying 257100,China)
出处
《天津医药》
CAS
北大核心
2021年第5期525-529,共5页
Tianjin Medical Journal
关键词
非酒精性脂肪性肝病
肝细胞核因子4
多态性
单核苷酸
non-alcoholic fatty liver disease
hepatocyte nuclear factor 4
polymorphism,single nucleotide
