摘要
【目的】研究喂食不同细菌后,家蚕不同组织ALP酶活性变化规律,明确ALP在家蚕抵御细菌侵染过程中发挥的功能和作用,为揭示ALP在昆虫体内的生理功能及选育抗细菌病蚕品种提供借鉴和思考。【方法】分别经口喂食大肠杆菌和金黄色葡萄球菌,采用微量酶标法检测家蚕血淋巴、中肠、马氏管和脂肪体ALP酶活性变化情况。【结果】中肠组织ALP酶活性在喂食不同细菌6 h就开始逐渐增加,在12 h达到最高值,随后急剧减弱,在24 h略低于对照组,而在48 h是极显著低于对照组水平。血淋巴ALP酶活性仅在喂食不同细菌24和48 h极显著高于对照组。喂食大肠杆菌和金黄色葡萄球菌3和6 h,马氏管ALP酶活性无显著变化,从12 h酶活性开始增强,24 h酶活性极显著高于对照组;而在48 h酶活性受到抑制,显著低于对照组。不论是大肠杆菌处理组还是金黄色葡萄球菌处理组,家蚕脂肪体ALP酶活性逐渐受到抑制,在12 h开始显著低于对照组,并随着处理时间的推移持续减弱,在24和48 h酶活性极显著低于对照组。【结论】大肠杆菌和金黄色葡萄球菌侵染家蚕,脂肪体ALP酶活性受到一定程度的抑制,而中肠、马氏管和血淋巴ALP酶活性呈现先显著升高,后急剧降低的变化趋势,暗示家蚕中肠、马氏管和血淋巴ALP可能参与了蚕体抵抗细菌侵染的免疫防御反应。
【Objective】In order to provide reference and thinking for revealing the physiological functions of ALP in insects and breeding silkworm varieties resistant to bacterial diseases,we studied the changes of alkaline phosphatase(ALP)activity in different tissues of silkworm after feeding different bacteria,and clarified the function and role of ALP in the process of resisting bacterial infection.【Method】The changes of ALP enzyme activity were detected by micro-enzyme assay in hemolymph,midgut,malpighian tubule and fat body of silkworm larvae after feeding Escherichia coli(E.coli)and Staphylococcus aureus.【Result】In the midgut,the ALP enzyme activity was gradually increased at 6 hours after feeding the E.coli and the Staphylococcus aureus,reached a maximum value at 12 hours,then decreased sharply and was slightly lower than that of the control group at 24 hours,and was significantly lower than that of the control group at 48 hours after feeding the E.coli or the Staphylococcus aureus.The hemolymph ALP enzyme activity was significantly higher than the control group only at 24 and 48 hours with the E.coli treatment group and the Staphylococcus aureus treatment group.There was no significant change of ALP enzyme activity in malpighian tubule at 3 and 6 hours after feeding different bacteria,the enzyme activity began to increase at 12 hours and was significantly higher than the control group at 24 hours,but the ALP enzyme activity was significantly lower than that of the control group after 48 hours.Both with the E.coli treatment and Staphylococcus aureus treatment,the ALP activity was gradually inhibited in fat body of silkworm and significantly lower than that of the control group at 12 hours and continued to weaken with the treatment time,and was significantly lower than that of the control group at 24 and 48 hours.【Conclusion】Whether E.coli or Staphylococcus aureus infected the silkworm,the ALP activity showed a trend of markedly increasing first and then sharply decreasing in midgut,malpighian tubule
作者
丁志伟
杨大平
王永生
杨伟克
高建华
DING Zhi-wei;YANG Da-ping;WANG Yong-sheng;YANG Wei-ke;GAO Jian-hua(Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences,Yunnan Mengzi 661101,China)
出处
《西南农业学报》
CSCD
北大核心
2021年第4期911-914,共4页
Southwest China Journal of Agricultural Sciences
基金
云南省现代农业蚕桑产业技术体系建设项目(2019 KJTX006)。
关键词
大肠杆菌
金黄色葡萄球菌
家蚕
碱性磷酸酶
酶活性
Escherichia coli
Staphylococcus aureus
Bombyx mori
Alkaline phosphatase
Enzymatic activity
