摘要
目的:测定妊娠期糖尿病(GDM)患者血浆miR-197表达水平及其与临床指标的相关性。方法:选取2019年1—6月在本院就诊的GDM患者84例、糖耐量正常孕妇84例和糖耐量异常孕妇50例。采用RT-qPCR法检测各组孕妇血浆miR-197水平,放射免疫法及葡萄糖氧化酶法检测各组空腹胰岛素(FINS)及空腹葡萄糖(FPG)水平。应用稳态模型法计算胰岛素抵抗指数(HOMA-IR)。Spearman相关性分析血清miR-197与FINS、FPG、HOMA-IR的相关性;ROC曲线分析miR-197预测GDM的敏感性及特异性。结果:GDM患者miR-197的相对表达量(1.86±0.13)低于正常孕妇(7.02±0.44)和糖耐量异常孕妇(4.595±0.20)(F=78.00,P<0.001)。相关分析显示,GDM组miR-197的表达与FPG、FINS、HOMA-IR均呈负相关(r=-0.862、-0.874、-0.939,P=0.01),miR-197预测GDM的敏感度(79.1%)和特异度(88.1%),其ROC曲线下面积为0.924(95%CI:0.890~0.957,P=0.00)。结论:血浆miR-197在GDM患者中低表达,其水平与血糖相关指标密切相关,有望成为临床诊断GDM的潜在指标。
Objective:To determine the expression level of miR-197 in plasma of women with gestational diabetes mellitus(GDM),and to study its correlation with clinical indicators of the women.Methods:84 pregnant women with GDM were selected in group A,50 pregnant women with abnormal glucose tolerance were selected in group B,and 84 pregnant women with normal glucose tolerance were selected in group C from January to June 2019.RT-qPCR was used to detect maternal plasma miR-197 level.The fasting glucose(FPG)and fasting insulin(FINS)levels were detected by radioimmunoassay and glucose oxidase methods.The insulin resistance index(HOMA-IR)was calculated by steady-state model.Spearman correlation analysis was used to study the correlation of serum miR-197 level with the levels of FINS and FPG,and HOMA-IR value.ROC curve was used to analyze the sensitivity and the specificity of the miR-197 level for predicting GDM.Results:The relative expression level of miR-197 of the women in group A(1.86±0.13)was significant lower than that(7.02±0.44)of the women in group C and that(4.595±0.20)of the women in group B(F=78.00,P<0.001).Correlation analysis showed that the expression of miR-197 level of the women in group A was negatively correlated with their FPG level,FINS level,and HOMA-IR value(r=-0.862,-0.874,-0.939,P=0.01).The sensitivity,the specificity,and ROC area of miR-197 level for predicting GDM were 79.1%,88.1%,and 0.924(95%CI:0.890-0.957,P=0.00).Conclusion:miR-197 level of the pregnant women with GDM is low,which is closely related to blood glucose-related indicators,so it is expected to be a potential indicator for diagnosing GDM.
作者
刘红雪
韩笑
张慕玲
LIU Hongxue;HAN Xiao;ZHANG Muling(The Affiliated Huai’an NO.1 People's Hospital of Nanjing Medical University, Huai’an, Jiangsu Province, 223300)
出处
《中国计划生育学杂志》
2021年第2期362-364,369,共4页
Chinese Journal of Family Planning
