摘要
【目的】克隆烟草木质素生物合成途径调控基因NtMYB42,为烟草品质改良提供参考依据。【方法】利用同源克隆法从栽培烟草K326克隆出NtMYB42基因全长序列和cDNA序列,研究分析NtMYB42基因的结构、编码蛋白理化性质、亚细胞定位和同源性等。【结果】NtMYB42基因组和cDNA全长分别为2312 bp和1130 bp,包含2个外显子和1个内含子,编码281个氨基酸,分子量为31.79 kD,等电点pI为5.18,预测有3个糖基化位点和18个磷酸化位点;NtMYB42与SlMYB42等植物MYB42蛋白同源性水平较高,与SlMYB42一致性达82.6%。【结论】从K326中克隆出NtMYB42基因全长序列和cDNA序列,NtMYB42是番茄SlMYB42、矮牵牛PnODO1和拟南芥AtMYB42的同源基因,是烟草木质素合成途径调控的重要候选基因。
【Objective】In order to provide a reference for quality improvement of tobacco,the tobacco lignin biosynthesis pathway regulation gene NtMYB42 is cloned.【Method】The full length sequence and cDNA sequence of NtMYB42 gene are cloned from cultivated tobacco K326 by using the homologous cloning method to study the structure,encoded protein physical-chemical properties,subcellular localization and homology of NtMYB42.【Result】The full-length of genome and cDNA in NtMYB42 are 2312 bp and 1130 bp respectively,containing two exons and one intron.NtMYB42 encodes 281 amino acids with molecular weight of 31.79 kD and isoelectric point of 5.18.There are 3 glycosylation sites and 18 phosphorylation sites.NtMYB42 has a high MYB42 homologous level with SlMYB42,and the consistency with SlMYB42 is up to 82.6%.【Conclusion】The full length sequence and cDNA sequence of NtMYB42 gene are cloned from K326.NtMYB42 is the homologous gene of SlMYB42 of tomato,PnODO1 of petunias and AtMYB42 of Arabidopsis,which is the key candidate gene for regulating tobacco lignin synthesis pathway.
作者
张程涵
赵会纳
余婧
余世洲
许本波
雷波
ZHANG Chenghan;ZHAO Huina;YU Jing;YU Shizhou;XU Benbo;LEI Bo(College of Life Science,Yangtze University,Jingzhou,Hubei 434025;Molecular Genetics Key Laboratory of China Tobacco,Guizhou Academy of Tobacco Science,Guiyang,Guizhou 550081,China)
出处
《贵州农业科学》
CAS
2021年第2期1-6,共6页
Guizhou Agricultural Sciences
基金
中国烟草总公司贵州省公司科技项目(201702)。
关键词
烟草
转录因子
NtMYB42
基因克隆
Nicotiana tabacum L
transcription factor
NtMYB42
gene cloning
