摘要
目的观察研究葛根素对骨质疏松大鼠氧化应激反应、骨代谢和骨密度的影响,探讨葛根素防治骨质疏松症的作用机制。方法44只雌性SD大鼠随机分对照组、造模组、雌激素组、葛根素组,采用“双侧卵巢摘除手术法”构建骨质疏松大鼠动物模型并给予相应药物干预,对照组和造模组:0.9%NaCl 5 mL/kg,葛根素组:葛根素35 mg/kg,雌二醇组:雌二醇150μg/kg,1次/d,皮下注射,连续给药6周。检测血清和骨组织氧化应激指标,检测骨代谢指标和骨组织BMD,观察骨组织形态学变化。结果造模组大鼠血清和骨组织SOD和GSH-Px较对照组显著降低(P<0.05),雌二醇组和葛根素组SOD和GSH-Px较造模组均显著升高(P<0.05);造模组大鼠血清和骨组织H2O2和MDA较对照组显著升高(P<0.05),雌二醇组和葛根素组H2O2和MDA较造模组均显著降低(P<0.05)。造模组大鼠血清TRACP5b、RANKL、PINP和BGP较对照组显著升高(P<0.05),雌二醇组和葛根素组骨代谢指标较造模组均显著降低(P<0.05)。造模组骨组织BMD较对照组显著降低(P<0.05),雌二醇组和葛根素组BMD较造模组均显著升高(P<0.05)。造模组骨皮质明显变薄,骨质疏松改变,骨小梁数量减少,排列紊乱,髓腔明显扩大,骨细胞明显减少。葛根素组和雌激素组新生骨小梁数量增多,间隙稍小,排列较规则,连接成网。结论葛根素通过调控骨质疏松大鼠氧化应激反应,改善骨代谢相关指标,提高骨密度,改善骨组织形态学结构,发挥抗骨质疏松作用。
Objective To observe the effect of puerarin on oxidative stress response,bone metabolism and bone mineral density in osteoporotic rats,and to explore the mechanism of action of puerarin on the prevention and treatment of osteoporosis.MethodsForty-four female SD rats were randomly divided into control group,model group,estrogen group,and puerarin group.Rat osteoporosis model was established with bilateral ovariectomy.Corresponding drug intervention was applied.Rats in control group and model group received 0.9%saline 5 mL/kg.Rats in estradiol group and puerarin group received subcutaneous injection of estradiol 150μg/kg and puerarin 35 mg/kg,respectively,1 time/day,for 6 weeks.Serum and bone tissue oxidative stress indicators and bone metabolism indicators were detected.BMD was examined and bone morphology changes were observe.Results SOD and GSH-Px in serum and bone tissue in model group were significantly lower than those in control group(P<0.05).SOD and GSH-Px in serum and bone tissue in estradiol group and puerarin group were significantly higher than those in model group(P<0.05).H2O2 and MDA in serum and bone tissue in model group were significantly higher than those in control group(P<0.05).H2O2 and MDA in estradiol group and puerarin group were significantly lower than those in model group(P<0.05).The serum levels of TRACP5b,RANKL,PINP,and BGP in model group were significantly higher than those in control group(P<0.05).The bone metabolism indexes in estradiol group and puerarin group were significantly lower than those in model group(P<0.05).BMD of bone tissue in model group was significantly lower than that in control group(P<0.05).BMD in estradiol group and puerarin group was significantly higher than that in model group(P<0.05).The cortical bone in model group was significantly thinner as osteoporosis change,the number of trabeculae decreased,the arrangement was disordered,the medullary cavity was significantly enlarged,and the bone cells were significantly reduced.In puerarin group and estrogen group,t
作者
杨占华
郝连升
张建新
YANG Zhanhua;HAO Liansheng;ZHANG Jianxin(Department of Orthopedics, Liaocheng Traditional Chinese Medicine Hospital, Liaocheng 252000, China;Department of Orthopedics, Shandong Provincial Hospital of Traditional Chinese Medicine, Jinan 250011, China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2021年第3期413-417,共5页
Chinese Journal of Osteoporosis
关键词
葛根素
氧化应激
骨代谢
骨密度
骨质疏松症
puerarin
oxidative stress
bone metabolism
bone mineral density
osteoporosis
