摘要
现存丙酮酸(Pyruvate, Py)检测方法大都需要特殊仪器,并且受某些适用范围的影响而存在缺乏选择性等问题。提出一种基于荧光银纳米簇(Ag nanoclusters, AgNCs)构建纳米传感平台定量精确检测Py的方法,建立了AgNCs的荧光发射强度与Py浓度线性定量关系,标准曲线方程为y=116.714+0.306x,R^(2)=0.991,Py浓度线性检测范围为200~1 400μmol/L。同时实验结果表明人体血液、尿液中的常见成分如葡萄糖(Glucose, GO)、尿酸(Uric acid, UA)、肌氨酸(Sarcosine, SO)、乳酸(Lactic acid, LA)等不会干扰该平台对于Py的测定。该方法具有很强的特异针对性,可实现对Py含量的高灵敏度和高选择性检测。
Most of the pyruvate(Py) detection methods require special instruments, and lack of selectivity due to the effect of certain application ranges.A method for quantitative and accurate detection of Py based on fluorescent silver nanoclusters(AgNCs) to construct a nano-sensing platform was presented.The linear quantitative relationship between the fluorescence intensity of AgNCs and the Py concentration has been established.The standard curve equation is y=116.714+0.306x,R^(2)=0.991,and the linear detection range of Py concentration is 200~1 400 μmol/L.Moreover, the results show that common components in human blood and urine such as glucose(GO),uric acid(UA),sarcosine(SO),lactic acid(LA),etc.Barely interfere the platform for the determination of Py.Therefore, the method has strong specificity and can realize high sensitivity, as well as high selectivity detection of Py content.
作者
肖利智
闫茹月
米继强
张阳
庞博
王淑敏
姜英男
XIAO Li-zhi;YAN Ru-yue;MI Ji-qiang;ZHANG Yang;PANG Bo;WANG Shu-min;JIANG Ying-nan(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《化学试剂》
CAS
北大核心
2021年第4期423-427,共5页
Chemical Reagents
基金
中央政府引导地方科技发展基金基础研究专项项目(202002055JC)
长春中医药大学科研开发基金项目“青培计划”。
关键词
丙酮酸检测
银纳米簇
荧光传感
线性定量
纳米探针
pyruvate detection
silver nanoclusters
fluorescence sensing
linear quantification
nano probe
