摘要
【目的】探究甘蔗间作猫豆对甘蔗根际土壤微生物多样性的影响,为改善甘蔗根际土壤微生物群落结构及促进甘蔗生长提供理论参考。【方法】采用单因素试验设计,分析单作甘蔗(CK)和甘蔗间作猫豆(T处理)2种种植方式对甘蔗根际土壤养分的影响,并采用Illumina HiSeq高通量测序技术,在门和属水平上分析甘蔗根际土壤微生物群落结构,在种水平进行主成分(PCA)分析。【结果】T处理的根际土壤中全氮、全钾、水解性氮、有效磷和有机质含量均大于CK。T处理的根际土壤中细菌的丰富度和多样性高于真菌的丰富度和多样性;T处理的根际土壤中细菌丰富度高于CK,但差异不显著(P>0.05,下同),其多样性显著高于CK(P<0.05,下同);T处理的根际土壤中真菌的丰富度和多样性均显著高于CK。CK和T处理土壤样品的主要优势细菌门为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteria),其相对丰度均大于10.00%,优势细菌属分别为水恒杆菌属(Mizugakiibacter)、游动四孢属(Luedemannella)、乳杆菌属(Acidothermus)和布氏杆菌属(Bryobacte),但T处理根际土壤样品中水恒杆菌属、游动四孢属和乳杆菌属的相对丰度分别较CK下降2.47%、1.78%和0.68%,其他细菌属分别增加0.10%~0.86%。CK和T处理根际土壤中优势真菌门为子囊菌门(Ascomycota),分别为80.00%和92.00%,其次是担子菌门(Basidiomycota),分别为8.00%和6.00%,排名前3的优势真菌属均为戴氏霉属(Taifanglania)、毛壳属(Chaetomium)和镰刀菌属(Fusarium)。CK和T处理的6份根际土壤样品在种水平上的主成分(PCA)分析结果显示,细菌群落PC1的变异为29.37%,PC2的变异为20.26%,二者的总贡献率为49.63%;真菌群落PC1的变异为33.44%,PC2的变异为27.73%,二者的总贡献率为56.17%。CK与T处理的细菌群落分别属于不同象限,CK位于第1和第3象限,而T处理位于第2和第4象限。【�
【Objective】The effects of Mucuna pruriens var.utilis intercropping with sugarcane on the microbial diversity in the rhizosphere of sugarcane were investigated,and a theoretical foundation was laid for improving the soil microbial community structure of M.pruriens intercropping with sugarcane and promoting the growth of sugarcane.【Method】A single-factor design experiment was conducted to analyze the microbial community structure of sugarcane rhizosphere soil at the phylum and genus levels by Illumina HiSeq high-throughput sequencing technology under the two planting methods of sugarcane monoculture(CK)and sugarcane intercropping M.pruriens(T treatment),and principal component analysis(PCA)to determine the effect of intercropping M.pruriens on sugarcane rhizosphere soil nutrients.【Result】The contents of total nitrogen,total potassium,hydrolyzable nitrogen,available phosphorus and organic matter in rhizosphere soil of T treatment were higher than those of CK treatment.The richness and diversity of bacteria in the rhizosphere soil of T treatment was higher than that of fungi.The bacterial richness of bacteria in the rhizosphere soil of Ttreatment was higher than that of CK,but the difference was not significant(P>0.05,the same below),its diversity was significantly higher than that of CK(P<0.05,the same below).The abundance and diversity of bacteria in the rhizosphere soil under T treatment were significantly higher than that of CK.The dominant phylum of bacteria in CK and T treatments were Proteobacteria,Actinobacteria,Chloroflexi and Acidobacteria,and their relative abundances were more than 10.00%.The dominant bacteria genera were Mizugakiibacter,Luedemannella,Actinothermus and Bruyobacte,but the relative abundances of Mizugakiibacter,Luedemannella,Actinothermus decreased by 2.47%,1.78%and 0.68%,other bacteria increased by 0.10%-0.86%compared to CK under T treatment.The dominant fungal phyla in the rhizosphere soil of CK and T treatments was Ascomycota,80.00%and 92.00%respectively,followed by Basidiomy
作者
毛莲英
李海碧
桂意云
张荣华
杨荣仲
周会
韦金菊
刘昔辉
MAO Lian-ying;LI Hai-bi;GUI Yi-yun;ZHANG Rong-hua;YANG Rong-zhong;ZHOU Hui;WEI Jin-ju;LIU Xi-hui(Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences/Sugarcane Research Center,Chinese Academy of Agricultural Sciences/Key Laboratory of sugarcane genetic improvement in Guangxi/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture and Rural Affairs,Nanning 530007,China;Guangxi South Subtropical Agricultural Science Research Institute,Longzhou,Guangxi 532415,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2021年第2期332-340,共9页
Journal of Southern Agriculture
基金
国家自然科学基金项目(31860350)
国家糖料产业技术体系建设专项(CARS-170105)
中央引导地方项目(桂科ZY20198005)
广西农业科学院基本科研业务专项(桂农科2021YT006)。
关键词
甘蔗
猫豆
间作
根际
土壤微生物
高通量测序
群落结构
sugarcane
Mucuna pruriens var.utilis
intercropping
rhizosphere
soil microbe
high-throughput sequencing
community structure
