摘要
目的:探讨腰椎间盘突出症(lumbar disc herniation,LDH)患者髓核组织中受体相互作用的丝氨酸苏氨酸激酶1(receptor-interacting protein serine-threonine kinases 1,RIPK1)表达和临床意义。方法:选取2016年1月至2018年1月治疗40例LDH患者的髓核组织标本为病例组,另选取同期行手术治疗30例腰椎骨折患者的髓核组织为对照组,分别采用聚合酶链反应(polymerase chain reaction,PCR),Western blot法检测两组患者髓核组织中RIPK1 mRNA和蛋白的表达,采用免疫组织化学染色法检测两组患者髓核组织中RIPK1蛋白表达,采用ELISA法检测两组患者髓核组织中RIPK1和TNF-α浓度,采用单因素方差分析髓核组织中RIPK1和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)浓度与LDH患者Pearce分级间的关系,采用Pearson分析RIPK1与TNF-α浓度间的相关性。结果:RIPK1在对照组髓核组织中呈弱阳性表达,RIPK1蛋白在病例组中呈阳性或强阳性表达。病例组髓核组织RIPK1 mRNA表达量高于对照组(P<0.05)。病例组髓核组织中RIPK1蛋白表达量高于对照组(P<0.05)。病例组髓核组织RIPK1浓度高于对照组(P=0.012)。病例组髓核组织TNF-α浓度显著高于对照组(P=0.009)。不同Pearce分级的LDH患者髓核组织中RIPK1和TNF-α浓度存在显著性差异(P>0.05),髓核组织中RIPK1和TNF-α浓度随着Pearce分级增加而显著升高。Pearson相关分析示LDH患者髓核组织中RIPK1和TNF-α浓度间存在显著正相关(r=0.781,P<0.001)。结论:LDH患者椎间盘组织中RIPK1 mRNA和蛋白表达水平高于正常的椎间盘组织,随着Pearce分级增高而升高,其可能是参与LDH炎性病变的重要因子。
Objective:To investigate the expression and clinical significance of receptor-interacting protein serine-threonine kinases 1(RIPK1)in the nucleus pulposus of patients with lumbar disc herniation(LDH).Methods:Nucleus pulposus tissue specimens of 40 patients with LDH patients underwent surgical treatment from January 2016 to January 2018 as the case group,and nucleus pulposus tissue specimens of 30 patients with lumbar spine fracture underwent surgical treatment at the same time as the control group.The expression of RIPK1 mRNA and protein of receptor interaction were detected by polymerase chain reaction(PCR)and Western blot,respectively.The expression of RIPK1 protein in the nucleus pulposus were detected by immunohistochemical staining.The concentrations of RIPK1 and tumor necrosis factor-α(TNF-α)in nucleus pulposus were detected by ELISA method.The relationship between the concentrations of RIPK1,TNF-αin nucleus pulposus and the Pearce grade of LDH patients was analyzed by one-way ANOVA.The correlation between RIPK1 and TNF-αwas analyzed by Pearson.Results:RIPK1 was weakly positively expressed in nucleus pulposus of control group,and RIPK1 protein was positively or strongly positively expressed in case group.The expression of RIPK1 mRNA in nucleus pulposus of case group was higher than that of control group(P<0.05).The expression of RIPK1 protein in nucleus pulposus of case group was higher than that of control group(P<0.05).The RIPK1 concentration in nucleus pulposus of case group was higher than that of control group(P=0.012).The concentration of TNF-αin nucleus pulposus of case group was significantly higher than that of control group(P=0.009).There were significant differences in concentrations of RIPK1 and TNF-αin nucleus pulposus of LDH pa tients with different Pearce classification(P>0.05).The concentration of RIPK1 and TNF-αin nucleus pulposus increased significantly with the increase of Pearce grade.Pearson correlation analysis showed that there was a significant positive correlation between RIP
作者
吴茂聪
王令玲
邓信昌
WU Mao-cong;WANG Ling-ling;DENG Xin-chang(Department of Spinal Surgery,Maoming People's Hospital,Southern Medical University,Maoming 525000,Guangdong,China)
出处
《中国骨伤》
CAS
CSCD
2021年第4期363-367,共5页
China Journal of Orthopaedics and Traumatology
基金
茂名市科技计划项目(编号:190326211708876)。
