摘要
目的分析核酸实验室2016~2019年环境监控情况,评价核酸实验室环境对血液筛查的影响。方法通过空气沉降、物表擦拭等方法,定期对NAT实验室进行月/季度环境监测,监控结果结合NAT联检/混检阳性率、鉴别/拆分阳性率进行统计分析和综合评价。结果 2016~2019年本中心对核酸实验室内外进行48批次环境监测,有11批共计25处反应性位点,其中15处(60%)为走廊门把手或门禁,系与血清学实验室的公共区域,加强清洁消毒后连续6个月未出现反应性,2017年11月出现核酸实验室内生物安全柜空气沉降阳性和Cobas S 201提取仪外部擦拭阳性,其余均为各区域门把手监测点阳性。Pearson相关性分析显示,环监不合格率与检测量、NAT联检/混检阳性率、鉴别/拆分阳性率均无相关性,无统计学意义。结论偶发的环境污染与检测量及阳性率无相关性,提示实验室现行的清消方法和防污措施及时有效,保证了检测结果的准确可靠。血液筛查实验室应根据实验室的布局和环境,逐步建立和完善环境监控体系,以实现核酸检测对环境的控制和要求。
Objective To analyze the environmental monitoring results of NAT laboratory from 2016 to 2019 and evaluate the influence of environmental quality on blood screening. Methods Monthly/Quarterly environmental monitoring was carried out in NAT laboratory regularly by means of air deposition and surface wiping. The monitoring results were statistically analyzed and comprehensively evaluated in combination with the pool/multiplexN ATreactiverate and discriminatory/resolutionrate.Results A total of 48 batches of environmental monitoring tests were conducted from 2016 to 2019,11( 25 reactive sites) were unqualified,including 15 reactive sites( 60%) as the hallway doorknob or access control,which were the public areas shared with serology laboratory,and these sites were not reactive for 6 consecutive months after intensive cleaning and disinfection. In Nov.2017,the air deposition in biosafety cabinet and surface wiping of Cobas s 201 were reactive,and the restwere the doorknobs. Pearson correlation analysis showed that there was no correlation between the positive rate of environmental monitoring and the detection amount,the pool/multiplex NAT reactive rate and discriminatory/resolution rate. Conclusion The accidental environmental pollution in the laboratory is not related to the detection amount and positive rate,indicating that the cleaning and anti-pollution measures are timely and effective to ensure the accuracy and reliability of the screen results. NAT laboratory should gradually establish and improve the environmental monitoring system according to the layout and environment so that the environmental quality meets the testing conditions.
作者
周磊
邓雪莲
邹亚轩
臧亮
ZHOU Lei;DENG Xuelian;ZOU Yaxuan;ZANG Liang(Dalian Blood Center,Dalian 116001,China)
出处
《中国输血杂志》
CAS
2021年第3期299-302,共4页
Chinese Journal of Blood Transfusion
关键词
核酸检测
环境监控
清洁消毒
生物安全
NAT
environmental monitoring
cleaning and disinfection
biosafety
