摘要
利用多种生物信息学手段从玉米基因组鉴定CDPKs家族基因,并从染色体定位、表达水平、功能聚类、功能预测分析及利用实时荧光定量PCR方法分析高温胁迫下该家族基因的表达水平。结果表明,ZmCDPKs包含32个家族成员,分布在9条染色体上,相对分子质量13.6~62.9 kD,将玉米、拟南芥和水稻序列比对分析,得出形成了4个明显的分支的结论,暗示着它们在功能上存在着差异。从ZmCDPKs家族中筛选出ZmCDPK6基因响应高温胁迫,通过RNA-seq数据分析,推测ZmCDPK6在果皮和胚根中高度表达,并且还受到盐胁迫和低温胁迫诱导表达。
In this study,we used a variety of bioinformatics methods to identify CDPKs family genes from maize genome,and analyzed the expression level of CDPKs family genes under high temperature stress from chromosome location,expression level,functional clustering,functional prediction by using real-time fluorescent quantitative PCR.The results showed that ZmCDPKs contained 32 family members,were distributed on 9 chromosomes,and the relative molecular weight was 13.6 to 62.9 kD.The sequence alignment analysis of maize,Arabidopsis and rice showed that they formed 4 distinct branches,implying that there were differences in their functions.ZmCDPK6 gene was screened from ZmCDPK6 family in response to high temperature stress.Through RNA-seq data analysis,it was speculated that ZmCDPK6 was highly expressed in pericarp and embryonic root,and was also induced and expressed by salt stress and cold stress.
作者
张宇萍
杨少玉
胡秀丽
杨浩
ZHANG Yuping;YANG Shaoyu;HU Xiuli;YANG Hao(College of Life Science, Henan Agricultural University, Zhengzhou 450002, China)
出处
《河南农业大学学报》
CAS
CSCD
2021年第2期227-233,256,共8页
Journal of Henan Agricultural University
基金
河南省科技攻关项目(192102110004)。
关键词
玉米
钙依赖蛋白激酶
高温胁迫
生物信息学
maize
calcium dependent protein kinase
high temperature stress
bioinformatics
