摘要
直径>200μm的透明介质微球作为一种厚透镜可以提高显微镜成像的分辨率和放大率,是降低显微成本的有效方法。文章针对微球透镜的放大作用,在傍轴条件下,应用球面折射公式推导出透明介质微球焦点的位置,研究了当透明介质微球焦点到样品距离不同时的成实像和成虚像放大倍率,并且实验验证了透明介质微球增大分辨率和放大率的作用。结果表明:其放大率由微球焦点和样品的位置决定,分辨率则由焦点光斑的位置和直径决定,而两者都与微球的大小和折射率有关,其中透明硅胶微球辅助的显微图像的放大率是显微原图的10倍,而200μm的钛酸钡微球可以实现125 nm的超分辨。
A transparent dielectric microsphere with diameter greater than 200μm can be used as a thick lens to improve the resolution and magnification of microscope,which is an effective method to cut down the cost of microscope.In this paper,the focus position was inferred by the spherical refraction formula in the case of paraxial according to geometrical optics.Both real image and virtual image were studied at different distances between the focus and the sample theoretically and the increasing effect by the dielectric microsphere on the magnification and resolution was demonstrated by experiments.The results show that the magnification is determined by the position of the focus of the microsphere and the position of the sample and the resolution is determined by the position and diameter of the focus spot,both of which are related to the size and refractive index of the microsphere.The magnification of the image assisted by transparent dielectric microspheres is 10 times that of the original image.And the super-resolution of 125 nm can be achieved by 200μm BaTiO3 microspheres.
作者
张剑
边填轩
李辉
张炆涛
李萌萱
ZHANG Jian;BIAN Tianxuan;LI Hui;ZHANG Wentao;LI Mengxuan(School of Science, Shandong Jianzhu University, Jinan 250101, China)
出处
《山东建筑大学学报》
2021年第2期53-60,共8页
Journal of Shandong Jianzhu University
基金
山东建筑大学博士基金项目(XNBS1715)
山东建筑大学国家级创新创业项目(201910430029)。
关键词
介质微球
分辨率
放大率
dielectric microsphere
resolution
magnification
