摘要
沙化土壤作为土地荒漠化的重要过渡形式,实现其有效恢复对减缓土地荒漠化进程意义重大.本研究显示,丛枝菌根(arbuscular mycorrhizal,AM)真菌和生物炭已应用于沙化土壤改良过程,但二者联合对沙化土壤改良影响研究较少;此外,细菌和真菌群落在沙化土壤改良过程中的作用尚不清楚.采用温室盆栽试验的方法,分别设置对照处理(CK)、单独接种AM真菌处理(RI)、单独施加生物炭处理(BC)和二者联合改良处理(BC_RI),研究不同改良方式对小果白刺(Nitrariasi birica Pall.)菌根侵染率、生物量、矿质营养元素(N、P、K、Ca和Mg)含量及土壤有机碳、营养元素(全N、全P和全K)、水稳性团聚体组成影响.采用高通量测序技术,考察细菌和真菌群落在沙化土壤改良过程中的作用,结合多元分析手段,探究不同改良方式改良作用机制,旨在为合理有效改良沙化土壤提供基础数据和理论依据.结果表明,接种处理(RI和BC_RI)小果白刺根系均观察到明显的菌根侵染现象,但RI和BC_RI处理间菌根侵染率无显著性差异.与CK相比,RI处理显著增加了小果白刺地上部生物量和地上部N、K、Ca和Mg含量,BC和BC_RI处理显著增加了小果白刺地上部、根部生物量及N、P、K、Ca和Mg含量;BC_RI处理与RI和BC相比,根部生物量及P、K、Ca和Mg含量显著增加.与CK相比,BC和BC_RI处理显著增加了土壤有机碳含量,RI处理使得土壤全N含量显著增加了152.54%,BC处理使得土壤全P和全K含量分别显著降低了12.5%和18.18%.各处理0.25~0.05 mm粒径土壤团聚体比例最高,BC_RI处理能够显著促进大粒径(>0.25 mm)土壤团聚体形成.与CK相比,RI和BC_RI处理显著降低细菌、真菌群落Sobs指数和Shannon指数;各处理细菌及真菌菌门组成及丰度存在显著差异性.RDA及网络分析结果显示,AM真菌、生物炭及二者联合改良方式对土壤基质环境及土壤微生物群落结构影响显著,不同改良方�
Sandy soils are considered as a significant transition phase to desertification.The effective recovery of sandy soils is of great significance to mitigate the desertification process.Some studies have shown that arbuscular mycorrhizal(AM)fungi and biochar improved the sandy soil,but there have been very few studies regarding the combined effects of AM fungi and biochar amendments on sandy soil improvement.Additionally,the roles of the bacterial and fungal community during the process of sandy soil improvement remain unclear.A greenhouse pot experiment with four treatments,including a control(CK,no amendment),single AM fungiassisted amendment(RI),single biochar amendment(BC),and combined amendment(BC_RI,biochar plus AM fungi),was set up.This study investigated the effects of different amendment methods on the Nitrariasi birica mycorrhizal colonization,biomass,nutrient(N,P,K,Ca,and Mg)content,soil organic carbon,soil nutrient(TN,TP,and TK)content,and soil water-stable aggregate composition.High throughput sequencing technology was used to investigate the roles of the bacterial and fungal communities during the process of sandy soil improvement.Combined with multiple analysis methods,the improvement mechanisms of different amendment methods were explored.The aim was to provide basic data and scientific basics for reasonably and effectively improving sandy soils.The results indicated that a significant mycorrhiza colonization was observed in the inoculation(RI and BC_RI)treatments,but there was no substantial difference in the mycorrhiza colonization with the RI and BC_RI.Compared with the CK,the shoot biomass and shoot element(N,K,Ca,and Mg)contents were significantly increased in the RI,and the shoot element(N,P,K,Ca,and Mg)contents were significantly increased in the BC and BC_RI;compared with the RI and BC,the root biomass and the root element(P,K,Ca,and Mg)contents were significantly increased in the BC_RI.Compared with the CK,the soil organic carbon contents were significantly increased in the BC and BC_RI,the
作者
张哲超
杨久扬
郝百惠
郝利君
罗俊清
李雪
刁风伟
张璟霞
郭伟
ZHANG Zhe-chao;YANG Jiu-yang;HAO Bai-hui;HAO Li-jun;LUO Jun-qing;LI Xue;DIAO Feng-wei;ZHANG Jing-xia;GUO Wei(Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle,Inner Mongolia Key Laboratory of Grassland Ecology,School of Ecology and Environment,Inner Mongolia University,Hohhot 010021,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2021年第4期2066-2079,共14页
Environmental Science
基金
国家自然科学基金项目(31860170,41977113)
内蒙古自治区科技重大专项(ZDZX2018054)
内蒙古自然科学基金项目(2018MS04003)。
关键词
微生物群落
丛枝菌根真菌
生物炭
联合改良
沙化土壤
改良机制
microbial community
arbuscular mycorrhizal(AM)fungi
biochar
combined amendment
sandy soil
improvement mechanism
