摘要
培氟沙星(pefloxacin,PEF)广泛用于畜禽疾病的预防和治疗,常造成兽药残留,影响人体健康。因此,有必要对其构建有效快速的检测方法。实验采用溶剂热法合成具有时间分辨特性的荧光纳米材料NaYF_(4)∶Ce/Tb,并基于分子对接辅助设计PEF适配体的互补链。纳米金与发光纳米材料分别修饰在适配体、互补链上,通过碱基互补配对诱导2种纳米材料间发生时间分辨荧光共振能量转移(time-resolved fluorescence resonance energy transfer,TR-FRET),基于此原理构建基于生物传感PEF的新型检测方法。在最佳条件下,测得PEF的时间分辨荧光强度在质量浓度0.2~20μg/kg呈良好的线性关系(y=1567.3x+3956.3,R^(2)=0.9742),检测限为0.15μg/kg,测得牛奶中兽药残留加标回收率为73.81%~99.86%,表明该方法可用于实际食品样品中PEF的检测。
Pefloxacin(PEF)is widely used in the prevention and treatment of livestock and poultry diseases,which is frequently causing veterinary drug residues in food and thus affecting human health.Therefore,it is necessary to construct an effective and rapid detection method for PEF.NaYF_(4):Ce/Tb nanomaterial with time-resolved fluorescence properties was synthesized by using the solvent hot method in this study.Simultaneously,complementary strand(cDNA)of PEF aptamer was designed by computer aided molecular docking.Gold nanoparticles and luminescent nanomaterials were modified onto aptamer and cDNA respectively.Thereby time-resolved fluorescence resonance energy transfer(TR-FRET)between these two nanomaterials was induced in case of complementary base pairing.Based on this principle,the novel detection method of PEF in milk was constructed.Under the optimal experimental conditions,the linear range of detection was 0.2-20μ/kg(y=1567.3x+3956.3,R^(2)=0.9742).The recovery rates were 73.81%-99.86%.These results showed that the present method can be applied in the detection of PEF in the practical milk sample.
作者
宋亚宁
胡超琼
霍秋宇
王力均
陈祥贵
黄玉坤
SONG Yaning;HU Chaoqiong;HUO Qiuyu;WANG Lijun;CHEN Xianggui;HUANG Yukun(School of Food and Bioengineering,Xihua University,Chengdu 610039,China;Key Laboratory of Food Non Thermal Processing,Engineering Technology Research Center of Food Non Thermal Processing,Yibin Xihua University Research Institute,Yibin 644004,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第7期244-250,共7页
Food and Fermentation Industries
基金
国家自然科学基金青年科学基金项目(31801647)
四川省科技厅项目(2020YFN0151,2020YFN0153)
西华大学创新训练项目(201710623035)
西华大学“西华杯”大学生创新创业项目(2021128)。
