摘要
目的检测膀胱癌患者外周血中循环肿瘤细胞(CTCs)的表达情况,探究在膀胱癌手术前后动态监测CTCs的价值。方法选择2018年10月~2019年6月就诊于承德医学院附属医院泌尿外科的92例膀胱癌患者作为试验组,另外选择同期22例健康志愿者作为对照组,采用免疫磁珠阴性富集法结合荧光细胞化学染色技术(SE-iFISH)检测两组外周血中CTCs水平,绘制受试者工作曲线(ROC),确定cut-off值,分析外周血中CTCs与试验组肿瘤浸润程度、病理分级、肿瘤分期及多样性的关系。结果试验组CTCs阳性率高于对照组(91.30%vs 4.50%),差异有统计学意义(P<0.05);当cut-off值为1个/7.5 ml时,CTC诊断膀胱癌的敏感性和特异性分别为91.30%、95.50%;不同浸润程度、病理分级的膀胱癌患者CTCs阳性率比较,差异有统计学意义(P<0.05);不同年龄、性别、肿瘤分期、多样性的膀胱癌患者CTCs阳性率比较,差异无统计学意义(P>0.05)。结论CTCs可作为诊断膀胱癌的评价指标,具有潜在的临床应用价值,可作为监测膀胱癌治疗疗效的肿瘤标志物,且其表达与肿瘤的浸润程度、病理分级有一定关系。
Objective To detect the expression of circulating tumor cells(CTCs)in the peripheral blood of patients with bladder cancer,and to explore the value of dynamic monitoring of CTCs before and after bladder cancer surgery.Methods 92 patients with bladder cancer who were admitted to the Department of Urology,Affiliated Hospital of Chengde Medical University from October 2018 to June 2019 were selected as the experimental group.In addition,22 healthy volunteers during the same period were selected as the control group,and the CTCs levels in the peripheral blood of the two groups were detected by the immunomagnetic bead negative enrichment method combined with the fluorescent cytochemical staining technique(SE-iFISH).Draw receiver operating curve(ROC),determine cut-off value,and analyze the relationship between CTCs in peripheral blood and tumor infiltration degree,pathological grade,tumor stage and diversity of the experimental group.Results The positive rate of CTCs in the experimental group was higher than that in the control group(91.30%vs 4.50%),the difference was statistically significant(P<0.05);When the cut-off value was 1/7.5 ml,the sensitivity and specificity of CTC in diagnosing bladder cancer were 91.30%and 95.50%,respectively;comparison of the positive rates of CTCs in bladder cancer patients with different infiltration degrees and pathological grades,the difference was statistically significant(P<0.05);There was no significant difference in the positive rate of CTCs among bladder cancer patients of different ages,genders,tumor stages,and diversity(P>0.05).Conclusion CTCs can be used as an evaluation index for the diagnosis of bladder cancer and have potential clinical application value.They can be used as tumor markers to monitor the therapeutic effect of bladder cancer,and their expression has a certain relationship with the degree of tumor invasion and pathological grade.
作者
苗岳松
刘杰
刘昕
王志勇
MIAO Yue-song;LIU Jie;LIU Xin;WANG Zhi-yong(Department of Urology,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei,China)
出处
《医学信息》
2021年第8期72-75,共4页
Journal of Medical Information
关键词
循环肿瘤细胞
膀胱癌
肿瘤标志物
免疫磁珠阴性富集法
Circulating tumor cells
Bladder cancer
Tumor markers
Immunomagnetic bead negative enrichment method
