摘要
目的研究TGF-β1对SW620结肠癌细胞体外增殖、迁移和PD-L1表达的影响,探讨ERK、AKT在TGF-β1调节PD-L1表达中的作用。方法 MTT法检测TGF-β1对SW620细胞增殖能力的影响;细胞划痕方法检测TGF-β1对SW620细胞迁移能力的影响;Real-time PCR、Western blot和免疫细胞化学方法分别检测TGF-β1对SW620细胞PD-L1 mRNA和蛋白表达的影响;Western blot检测TGF-β1处理后SW620的ERK、AKT表达变化及抑制ERK、AKT后PD-L1的表达情况。结果 SW620细胞培养24h、TGF-β1浓度为0~20ng/ml时,细胞活性随浓度升高而增加;培养48h时TGF-β1为10ng/ml时细胞活性最强。TGF-β1处理后SW620迁移无明显变化,TGF-β1上调SW620细胞PD-L1 mRNA和蛋白表达水平,增强SW620细胞ERK、AKT蛋白磷酸化水平,抑制ERK和AKT后,TGF-β1促进PD-L1表达的作用受到抑制。结论 TGF-β1促进SW620细胞体外增殖,但不影响其迁移,促进SW620细胞表达PD-L1,且这种促进作用可能与ERK、AKT有关。
Objective To study the effect of TGF-β 1 on the proliferation, migration and PD-L1 expression in SW620 colon cancer cells, to explore the roles of ERK and AKT in the regulation of TGF-β1 on PD-L1 expression. Methods The cells proliferation and migration were detected by MTT and wound healing assays, respectively. Real-time quantitative PCR, Western blot and immunofluorescence were used to detect PD-L1 mRNA and protein and ERK and AKT expressions in TGF-β1 treated SW620 cells. Results TGF-β1 promoted significantly the proliferation of SW620 cells in a dose-dependent manner at the range of 0~20 ng/ml, the proliferation reached the peak at 10 ng/ml of TGF-β1, but with no effect on SW620 migration. TGF-β1 effectively increased expression level of PD-L1 m RNA and protein and phosphorylation level of ERK and AKT in SW620 cells. In addition, the promotion of TGF-β1 on PD-L1 expression was downregulated significantly by inhibition of ERK and AKT. Conclusion TGF-β1 enhanced the proliferation of SW620 cells and promoted the expression of PD-L1, which is related to ERK and AKT pathways.
作者
李卓伟
柯佳
李超
杨慧科
吕晓红
张雅芳
毛春梅
李雪梅
LI Zhuo-wei;KE Jia;LI Chao;YANG Hui-ke;LV Xiao-hong;ZHANG Ya-fang;MAO Chun-mei;LI Xue-mei(Department of Human Anatomy,Harbin Medical University,Harbin 150086;Pediatrics Department of the Fourth Affiliated Hospital,Harbin Medical University,Harbin 150001,China)
出处
《解剖科学进展》
CAS
2021年第1期90-94,共5页
Progress of Anatomical Sciences
基金
黑龙江省省属高等学校基本业务费科研项目。
