摘要
为了解不同培养基组分对牛支原体生长的影响,试验选用3种动物血清和4个厂商的琼脂,按照5%、10%、15%的血清浓度依次制备了9种液体培养基,分别进行了牛支原体生长试验;在此基础上,用4个厂商的琼脂依次制备了4种固体培养基,分别进行了牛支原体生长试验;通过对液体培养基OD值的测定和对固体培养基性能指标评价来判断牛支原体生长状况。结果表明,牛支原体在9种液体培养基中均能生长,且培养至60 h时其OD值达到最大;在添加血清浓度相同的情况下,添加马血清培养基的OD值与添加猪血清或胎牛血清培养基的OD值相比差异显著(P<0.05);在添加相同类型血清的情况下,添加猪血清或胎牛血清的各培养基之间的OD值相比差异不显著(P>0.05),而添加马血清的各培养基之间的OD值相比,添加量10%和15%与5%相比差异显著(P<0.05),而10%与15%相比差异不显著(P>0.05)。而固体培养基的指标性能评分分别为9(BD公司)、7(香港某公司)、5(上海某公司)、6分(成都某公司)。说明添加10%马血清的液体培养基最适宜牛支原体生长,固体培养基应在此液体培养基的基础上,添加BD公司的琼脂所制备的固体培养基最适宜牛支原体的生长。
In order to understand the effects of different medium components on the growth of Mycoplasma bovis,the experiment selected 3 animal serums and 4 manufacturers’agars,and prepared 9 liquid media in sequence at 5%,10%,and 15%serum concentrations.The growth test of Mycoplasma bovis was carried out;On this basis,four solid mediums were prepared sequentially with agar from 4 manufacturers,and the growth test of Mycoplasma bovis was carried out respectively.Through the determination of the OD value of the liquid medium and the performance of the solid medium index evaluation to judge the growth status of Mycoplasma bovis.The results show that Mycoplasma bovis can grow in 9 kinds of liquid media,and its OD value reaches the maximum when it is cultured for 60 h;When the serum concentration is the same,the OD value of the medium supplemented with horse serum is the same as that of the supplemented porcine serum.The OD value of the fetal bovine serum medium is significantly different(P<0.05);When the same type of serum is added,the OD value of each medium supplemented with pig serum or fetal bovine serum is not significantly different(P>0.05),and compared with the OD value of each medium supplemented with horse serum,the addition amount of 10%and 15%is significantly different from 5%(P<0.05),while the difference between 10%and 15%is not significant(P>0.05).The index performance scores of solid media are 9 points(BD company),7 points(a company in Hong Kong),5 points(a company in Shanghai),and 6 points(a company in Chengdu).It indicates that the liquid medium supplemented with 10%horse serum is most suitable for the growth of Mycoplasma bovis.The solid medium should be based on this liquid medium,and the solid medium prepared by adding BD agar is the most suitable for the growth of Mycoplasma bovis.
作者
严明帅
徐业芬
张冯禧
索朗斯珠
牛家强
YAN Ming-shuai;XU Ye-fen;ZHANG Feng-xi;SUOLANG Si-zhu;NIU Jia-qiang(Animal Science College,Tibet Agriculture&Animal Husbandry University,Nyingchi 860000,Tibet,China;Provincial Key Laboratory of Tibet Plateau Animal Epidemic Disease Research,Nyingchi 860000,Tibet,China)
出处
《湖北农业科学》
2021年第7期99-102,105,共5页
Hubei Agricultural Sciences
基金
西藏自治区厅校联合基金(XZ2018ZRG-15)
国家肉牛牦牛产业体系项目(CARS-37)
西藏特色家畜选育与健康养殖专项(XZ201801NA03)。
关键词
牛支原体
培养基组分
性能指标
Mycoplasma bovis
medium composition
performance index
