摘要
目的:探讨酶联免疫法与化学发光法检测丙型肝炎病毒抗体的对比性研究及对特异度的影响。方法:选取2018年1-12月于本院进行丙型肝炎病毒抗体检查的患者568例,所有患者均进行酶联免疫法与化学发光法检测。分析重组免疫印迹法(RIBA)确证酶联免疫法结果,RIBA法确证化学发光法结果,比较酶联免疫法与化学发光法检测的准确度、灵敏度、特异度。结果:RIBA法确诊的阳性标本为11例(1.9%),酶联免疫法检查结果,阳性标本为65例(11.4%),化学发光法检查结果,阳性标本为34例(6.0%)。化学发光法准确度、特异度均高于酶联免疫法,两种方法差异有统计学意义(P<0.05);两种方法灵敏度比较,差异无统计学意义(P>0.05)。结论:丙型肝炎病毒抗体检测中,化学发光法的准确度、特异度高于酶联免疫法,通过化学发光法可以有效提高临床监测准确度,但是对酶联免疫法与化学发光法检测均存在可疑现象时,需使用RIBA再次进行确定。
Objective:To explore the comparative study on enzyme-linked immunosorbent assay(ELISA)and chemiluminescence(CL)in the detection of hepatitis C virus(HCV)antibody and its effect on specificity.Method:A total of 568 patients tested for hepatitis C virus antibody were selected from our hospital from January to December 2018.All patients were tested by ELISA and CL.Recombinant western blotting(RIBA)was used to confirm the results of ELISA,RIBA was used to confirm the results of CL.The accuracy,sensitivity and specificity of ELISA and CL were compared.Result:There were 11 cases(1.9%)positive specimens confirmed by RIBA,65 cases(11.4%)positive specimens confirmed by ELISA,the chemiluminescence were positive in 34 cases(6.0%).The accuracy and specificity of CL method were higher than that of ELISA,the differences between the two methods were statistically significant(P<0.05).There was no significant difference in the sensitivity between the two methods(P>0.05).Conclusion:In the detection of hepatitis C virus antibody,the accuracy and specificity of CL are higher than that of ELISA.CL can effectively improve the accuracy of clinical monitoring and reduce the missed diagnosis.However,if there are suspicious phenomena in the detection of ELISA and CL,RIBA should be used to determine again.
作者
孙健
SUN Jian(Heilongjiang Jiamusi Maternal and Child Health Hospital,Jiamusi 154004,China)
出处
《中国医学创新》
CAS
2021年第4期136-139,共4页
Medical Innovation of China
关键词
酶联免疫法
化学发光法
丙型肝炎病毒抗体
特异度
Enzyme-linked immunosorbent assay
Chemiluminescence
Hepatitis C virus antibody
Specificity
