摘要
【目的】探究薄荷(Mentha haplocalyx Brig).精油中可影响二化螟(Chilo suppressalis Walker)触角电位反应的化学物质。【方法】采用水蒸气蒸馏法提取薄荷精油,称重法测试薄荷精油对二化螟幼虫取食的影响,采用气相色谱-触角电位联用仪(GC-EAD)分析薄荷精油主要化学成分对二化螟成虫的电位反应。【结果】薄荷精油对二化螟幼虫具有显著的引诱取食作用,当浓度为0.15625 mg/mL时,取食率为123.01%。GC-MS结果分析从薄荷精油中共检测出27个化合物,化合物含量占总含量的81.965%,含量最多的为长叶薄荷酮,为15.854%。GC-EAD测定结果表明,雌虫对薄荷精油中的12种化合物作出反应,长叶薄荷酮的反应最为强烈;雄虫对薄荷精油中的10种化合物有反应,丁子香酚对二化螟雄虫的影响最为强烈。【结论】薄荷精油中的长叶薄荷酮和丁子香酚可能是影响二化螟幼虫取食行为的主要化合物。
【Objective】The study aims to investigate the chemical components of essential oil of Mentha haplocalyx Brig.affecting electroantennogram response of Chilo suppressalis Walker.【Method】The essential oil of M.haplocalyx was extracted by steam distillation.Then,the feeding behavioral response of C.suppressalis and the electroantennogram responses of the chemical components of M.haplocalyx essential oil were tested by using weight method and gas chromatography-electroantennographic detection(GC-EAD),respectively.【Result】The essential oil of M.haplocalyx had feeding attraction effect to C.suppressalis,and the feeding rate was 123.01%when the concentration was 0.15625 mg/mL.The results of GC-MS showed that 27 compounds were detected in the essential oil of M.haplocalyx,accounting for 81.965%of the total content.The most abundant compound was pulegone,accounting for 15.854%.GCEAD detection results showed that 12 compounds of essential oil of M.haplocalyx caused significant reaction in female adults of C.suppressalis,which showed the strongest reaction to pulegone.Significant reaction in male adults of C.suppressalis were observed in 10 compounds of essential oil of M.haplocalyx and that was the strongest in eugenol.【Conclusion】Pulegone and eugenol in essential oil of M.haplocalyx may be main compounds affecting the feeding behavior of C.suppressalis.
作者
杨雪
胡阳
王梓懿
王照国
余帅
安正斌
夏宇
李
YANG Xue;HU Yang;WANG Ziyi;WANG Zhaoguo;YU Shuai;AN Zhengbin;XIA Yu;LI Yan(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs,Guiyang 550014,China;Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)
出处
《广东农业科学》
CAS
2021年第3期98-104,共7页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31660530)
国家重点研发计划项目(2017YFD0201402-05)
贵州省科技支撑计划项目(黔科合〔2020〕1Y173)。
关键词
薄荷精油
成分分析
二化螟
取食行为
气相色谱-触角电位反应
水蒸气蒸馏法
essential oil of Mentha haplocalyx Brig
component analysis
Chilo suppressalis Walker
feeding behavior
gas chromatography-electroantennographic detection(GC-EAD)
steam distillation
