摘要
目的探究儿茶素基于MEK/ERK信号通路对老年冠心病大鼠内皮功能的作用机制。方法 2020年1—11月于湖北恩施州中心医院实验室进行实验。选取健康老年SD大鼠50只,随机选取10只为对照组,余40只建立老年冠心病模型,分为模型组,儿茶素小剂量组、中剂量组、高剂量组。造模后,模型组继续高脂喂食,小、中、高剂量组分别给予儿茶素20、40、60 mg/kg。对照组正常饮食。对比各组大鼠血清Hcy、血管内膜厚度、TNF-α、IL-1β、IL-6、NO、eNOS、ET-1、ADMA及MEK、ERK蛋白表达水平。结果与对照组比较,模型组大鼠血清Hcy、血管内膜厚度上升;与模型组比较,儿茶素小、中、高剂量组大鼠血清Hcy、血管内膜厚度依次下降(F/P=44.566/<0.001,36.852/<0.001);与对照组比较,模型组大鼠NO、eNOS水平下降,ET-1、ADMA、TNF-α、IL-1β、IL-6水平上升;与模型组比较,儿茶素小、中、高剂量组大鼠血清NO、eNOS水平依次上升,ET-1、ADMA、TNF-α、IL-1β、IL-6水平依次下降(F/P=51.231/<0.001,19.492/<0.001,35.267/<0.001,19.985/<0.001,44.410/<0.001,17.548/<0.001,45.365/<0.001);与对照组比较,模型组大鼠心肌MEK、ERK蛋白表达升高;与模型组比较,儿茶素小、中、高剂量组大鼠MEK、ERK蛋白表达依次下降(F/P=19.856/<0.001,45.385/<0.001)。结论儿茶素能改善老年冠心病大鼠血管内皮功能,调节MEK/ERK信号通路,抑制大鼠机体炎性因子。
Objective To explore the mechanism of catechins based on MEK/ERK signaling pathway on endothelial function in aged rats with coronary heart disease.Methods The experiment was conducted in the laboratory of Enshi Central Hospital from January to November 2020.50 healthy old SD rats were selected,10 rats were randomly selected as the control group,and the remaining 40 rats were established coronary heart disease model.They were divided into model group,low-dose group(20 mg/kg),medium-dose group(40 mg/kg)and high-dose group(60 mg/kg).After modeling,the model group continued to be fed with high-fat diet,and the low,medium and high-dose groups were given catechin 20,40 and 60 mg respectively.The control group received normal diet.The levels of serum Hcy,intimal thickness,TNF-α,IL-1β,IL-6,NO,eNOS,ET-1,ADMA,MEK and ERK protein were compared among the groups.Results Compared with the control group,the serum Hcy and vascular intima thickness of rats in the model group increased;compared with the model group,the serum Hcy and vascular intima thickness of rats in the low-dose,medium-dose,and high-dose groups decreased sequentially(F/P=44.566/<0.001,36.852/<0.001);Compared with the control group,the levels of NO and eNOS in the model group decreased,and the levels of ET-1,ADMA,TNF-α,IL-1β,and IL-6 increased;compared with the model group,the small,medium,and high-dose groups The levels of NO and eNOS in rats increased sequentially,and the levels of ET-1,ADMA,TNF-α,IL-1β,and IL-6 decreased sequentially(F/P=51.231/<0.001,19.492/<0.001,35.267/<0.001,19.985/<0.001,44.410/<0.001,17.548/<0.001,45.365/<0.001);Compared with the control group,the expression of MEK and ERK protein in myocardium of the model group increased;compared with the model group,MEK and ERK in the small,medium and high dose groups The protein expression decreased sequentially(F/P=19.856/<0.001,45.385/<0.001).Conclusion Catechin can improve the vascular endothelial function of elderly rats with coronary heart disease,regulate the MEK/ERK signal pathway,and
作者
周慧
雷玉华
张复贵
杨淑蓉
华晓芳
黄晶
Zhou Hui;Lei Yuhua;Zhang Fugui;Yang Shurong;Hua Xiaofang;Huang Jing(Cardiovascular Center,Enshi Central Hospital,Hubei Province,Enshi 445000,China)
出处
《疑难病杂志》
CAS
2021年第4期353-357,共5页
Chinese Journal of Difficult and Complicated Cases
基金
湖北省自然科学基金资助项目(20150916)。
关键词
儿茶素
冠心病
内皮功能
炎性因子
老年
大鼠
Catechins
Coronary heart disease
Endothelial function
Inflammatory factors
Elderly
Rats
