摘要
目的探讨藏族冠心病患者肠道短链脂肪酸与肠道菌群、血脂的相关性,为藏族冠心病患者临床个体化治疗提供新思路。方法选取2017年8月至2018年2月青海省心脑血管病专科医院冠心病科收治的有冠心病症状的藏族疑似患者30例,根据CT和冠脉造影结果,将单支或多支冠脉狭窄≥50%的患者分为冠心病组(CHD组,18人),其余患者纳入非冠心病组(NCHD组,12人),并选取同期体检健康的藏族志愿者为健康藏族组(HT组,23人),采集3组研究对象空腹血清和清晨粪便样本。粪便行16S rDNA测序,全自动气相色谱-质谱联用仪检测短链脂肪酸含量,血清中血脂水平采用全自动血液生化仪检测。采用Pearman相关分析方法综合分析菌群、血脂与短链脂肪酸的关系。结果CHD组和NCHD组患者高密度脂蛋白胆固醇均低于HT组,NCHD组粪便异丁酸水平高于HT组,差异均有统计学意义(P<0.05)。与HT组相比,CHD组与NCHD组小类杆菌属(Dialister)相对丰度均升高,劳特氏菌属(Blautia)相对丰度降低。与NCHD组相比,CHD组拟杆菌属(Bacteroides)相对丰度降低,差异均有统计学意义(P<0.05)。Spearman相关分析显示,HDL水平与CHD组粪便异丁酸水平呈正相关(r=0.808,P<0.01)。HT组异丁酸水平与普氏菌属相对丰度呈负相关(r=-0.438,P<0.05),与劳特氏菌属相对丰度呈正相关(r=0.467,P<0.05)。NCHD组劳特氏菌属相对丰度与异丁酸水平呈正相关(r=0.627,P<0.05)。结论藏族冠心病患者肠道菌群组成、血脂及短链脂肪酸水平发生变化,异丁酸水平与菌群丰度、血脂水平相关。
Objective Tostudy the correlation of intestinal flora,short-chain fatty acids(SCFAs)and blood lipids in Tibetan patients with coronary heart disease for the individualized clinical treatment.Methods From August 2017 to February 2018,30 Tibetan with suspected CHD were hospitalized in the Department of Coronary Heart Disease in Qinghai Cardio-cerebrovascular Disease Special Hospital and enrolled in this study.According to the results of CT and coronary angiography,the inpatients with single or multiple coronary artery stenosis≥50%were divided into CHD group(CHD group,18 patients),and the remaining patients were included in patient control group(NCHD group,12 patients).Another 23 Tibetan volunteers served as the hea lthy control group(HT group).Fasting serum and fecal samples were collected from the subjects in the three groups.The faecal microbiota of all samples were profiled by 16S rDNA.The content of SCFAs was detected by a full-automatic gas chromatography-mass spectrometer,and the serum lipid level was detected by a full-automatic blood biochemical analyzer.The relationship between bacteria,blood lipids and short-chain fatty acidswas analysed by Spearman correlation analysis.Results High density lipoprotein cholesterol(HDL-c)in CHD group and NCHD group was lower than that in HT group,and the level of fecal isobutyric acid in NCHD group was higher than that in HT group,the differences were statistically significant(P<0.05).Compared with HT group,the relative abundance of Dialister increased in CHD group and NCHD group,while the relative abundance of Blautia decreased.Compared with NCHD group,the relative abundance of Bacteroides in CHD group decreased,and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that HDL-c level was positively correlated with fecal isobutyric acid level in CHD group(r=0.808,P<0.01).There was a negative correlation between isobutyric acid level and relative abundance of Prevotella in HT group(r=-0.438,P<0.05),and a positive correlation with th
作者
刘凤云
马焱
谈月琴
侯海文
李媛
李愈娴
LIU Fengyun;MA Yan;TAN Yueqing(Key Laboratory of High Altitude Medicine,National Health Commission of the People’s Republic of China/Qinghai Cardio-cerebrovascular Disease Special Hospital,Xining 810012,China)
出处
《安徽医学》
2021年第2期148-152,共5页
Anhui Medical Journal
基金
青海省基础研究项目(项目编号:2017-ZJ-720)。
关键词
藏族
冠心病
肠道菌群
短链脂肪酸
血脂
16S
rDNA
Tibetan
Coronary heart disease
Intestinal flora
Short-chain fatty acids
Blood lipid
16S rDNA
